Title (croatian) Preživljavanje legionela u morskoj vodi
Author Dijana Tomić Linšak
Author Andrea Gašaj
Author Ivana Gobin
Author's institution University of Rijeka Faculty of Medicine (Department of Microbiology and Parasitology)
Author's institution University of Rijeka Faculty of Medicine (Department of Health Care)
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Public Health and Health Care Health Ecology
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Medical Microbiology
Abstract (german) Legionellen kommen vor allem in künstlichen Wasserumgebungen vor, z.B. in Warmwasseranlagen, Jacuzzis, Schwimmbäder, Klimaanlagen u ä. Es ist auch gut bekannt, dass Legionellen in Süßwassersystemen vorkommen; ihr Überleben in der Umgebung mit hohen Salzkonzentrationen ist allerdings bisher nicht näher untersucht worden. In dieser Studie wurde anhand von In-vitro-Untersuchungen das Überleben von Legionellen in Proben von Meerwasser, Leitungswasser und gechlortem Wasser aus dem Meerschwimmbad geprüft. Zwei Bakterienstämme wurden verwendet, Legionella pneumophila ATCC BAA-74 als klinisches Isolat und Legionella pneumophila ST82, der aus dem Meerschwimmbadwasser isoliert wurde. In unbehandeltem Meerwasser sowie im Leitungswasser überleben die beiden Bakterienarten über 30 Tage. Im filtrierten Meerwasser überlebt L. pneumophila ST82 (Serotyp 1) über 30 Tage, während L. pneumophila ATCC BAA-74 innerhalb 25 Tage abstirbt. Ein wichtiger Überlebensfaktor bei beiden Bakterienstämmen ist die Verdünnung des Meerwassers mit nicht gechlortem Leitungswassers. Die Ergebnisse zeigen, dass eine ständige Kontrolle und Durchführung des Protokolls für Wasserqualität in Hotels notwendig sind, und dass zusätzliche Protokolle für Meerwasserschwimmbäder eingeführt werden sollen. Nur mit der Durchführung entsprechender Hygienemaßnahmen und Überwachungsprozesse lässt sich das Risiko einer Legionellose verringern.
Abstract (english) Legionellae primarily appear in water environments, mostly artificially created, such as warm water systems, Jacuzzis, recreation pools, air conditioning systems, etc. It is well known that legionellae survive in freshwater systems; however, their survival in water with high salt concentrations has not been researched in detail. In vitro tests in this study examined the survival of Legionella in samples of seawater, potable water and chlorinated water from a saltwater pool. We used two bacterial strains - Legionella pneumophila ATCC BAA-74 as the clinical isolate and Legionella pneumophila ST82 isolated from the saltwater pool.
Both bacteria survive over 30 days in raw seawater and potable water. In filtered seawater, L. pneumophila strain ST82 survives over 30 days, whereas L. pneumophila ATCC BAA-74 dies within 25 days. An important factor for the survival of both strains is a dilution of chlorinated seawater with non-chlorinated potable water. Our results indicate that a constant control and adherence to water managing protocols are essential in hotel systems, and that additional protocols for saltwater pools are necessary. Only adequate hygiene and monitoring processes can lead to a significant risk reduction of the occurrence and spread of Legionnaires’ disease.
Abstract (croatian) Legionele se primarno nalaze u vodenim sredinama, najčešće umjetno stvorenim, kao što su sustavi s toplom vodom, jacuzziji, rekreacijski bazeni, klima uređaji i slično. Dobro je poznata činjenica da legionele preživljavaju u slatkovodnim sustavima, no preživljavanje uz visoke koncentracije soli nije detaljno ispitano. U ovoj studiji in vitro pokusima ispitano je preživljavanje Legionella u uzorcima morske vode, vodovodne vode te klorirane vode iz morskog bazena. Korištena su dva soja bakterije, Legionella pneumophila ATCC BAA-74 kao klinički izolat i Legionella pneumophila ST82 koja je izolirana iz morskog bazena.
U sirovoj morskoj vodi, kao i u vodovodnoj vodi obje vrste bakterije preživljavaju više od 30 dana. U filtriranoj morskoj vodi L. pneumophila soj ST82 preživljava preko 30 dana, dok L. pneumophila ATCC BAA-74 ugiba unutar 25 dana. Važan faktor u preživljavanju oba soja je i razrjeđenje klorirane morske vode s nekloriranom vodovodnom vodom. Rezultati ukazuju da je neophodna konstantna kontrola i pridržavanje protokola održavanja voda u hotelskim sustavima, te je neophodno uvođenje dodatnih protokola za bazene s morskom vodom. Jedino adekvatnim procesima higijene i monitoringa možemo dovesti do značajnijeg smanjenja rizika od pojave legionarske bolesti i njenog širenja.
Keywords (german)
Legionella pneumophila, Meerwasser, Überleben von Bakterien
Keywords (english)
Legionella pneumophilla, seawater, bacterial survival
Keywords (croatian)
Legionella pneumophilla, morska voda, preživljavanje bakterija
Language croatian
Publication type Scientific paper - Original scientific paper
Publication status Published
Peer review Peer review
Publication version Published version
Journal title Hrvatske vode
Numbering vol. 29, no. 116, pp. 75-82
p-ISSN 1330-1144
e-ISSN 1849-0506
URN:NBN urn:nbn:hr:184:221310
Publication 2021
Document URL https://hrcak.srce.hr/261220
Type of resource Text
Access conditions Open access
Terms of use
Created on 2021-08-31 10:41:02