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master's thesis
UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM
Rijeka: University of Rijeka, Faculty of Medicine, 2021. urn:nbn:hr:184:250552
Lovrić, Lucija
University of Rijeka
Faculty of Medicine
Department of Pharmacology

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Lovrić, L. (2021). UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:250552

Lovrić, Lucija. "UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM." Master's thesis, University of Rijeka, Faculty of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:184:250552

Lovrić, Lucija. "UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM." Master's thesis, University of Rijeka, Faculty of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:184:250552

Lovrić, L. (2021). 'UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:184:250552

Lovrić L. UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2021 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:184:250552

L. Lovrić, "UČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:184:250552

Metadata
TitleUČINAK REKOMBINANTNOG ERITROPOETINA NA EKSPRESIJU I AKTIVNOST SIRT1 U MOZGU ŠTAKORA S EKSPERIMENTALNIM DIJABETESOM
Title (english)EFFECT OF RECOMBINANT ERYTHROPOIETIN ON THE BRAIN SIRT1 EXPRESSION AND ACTIVITY IN RATS WITH EXPERIMENTAL DIABETES MELLITUS
AuthorLucija Lovrić
MentorJasenka Mršić-Pelčić (mentor)
Committee memberKristina Pilipović (predsjednik povjerenstva)
Committee memberDinko Vitezić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Pharmacology)
Rijeka
Defense date and country2021-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Abstract
Dijabetes (Diabetes mellitus) je ozbiljna, za sada neizlječiva, kronična bolest koja je okarakterizirana hiperglikemijom kao i poremećajima u metabolizmu ugljikohidrata, masti i proteina. U zadnja dva desetljeća se drastično povećala incidencija ove bolesti, pretežito kod osoba starije dobne skupine, ali ne zaobilazi niti mlađu populaciju. Upravo zbog toga je sve više istraživanja usmjereno na razvoj novih, dostupnijih i učinkovitijih metoda terapijskog liječenja. U ovom diplomskom radu je ispitivan učinak rekombinantnog humanog eritropoetina (rhEPO) na ekspresiju i aktivnost SIRT1 proteina (engl. silent information regulator two protein 1), ekspresiju proBDNF i mBDNF proteina kao i na IkBα protein u parijatalnom korteksu i/ili hipokampusu štakora s eksperimentalnim dijabetesom. Hannover Wistar štakorima muškog spola je izazvan dijabetes primjenom pojedinačne injekcije streptozotocina (STZ). Dio životinja koje su razvile dijabetes primile su rhEPO (40 µg/kg tjelesne težine) (Diab/EPO) ili otapalo (Diab) četiri dana uzastopno. Kontrolnu skupinu sačinjavale su zdrave, netretirane životinje. Dobiveni rezultati pokazuju da je primjena rhEPO izazvala statistički značajan porast ekspresije SIRT1 proteina u mozgovima štakora s eksperimentalnim dijabetesom u korteksu i hipokampusu u usporedbi sa kontrolnom skupinom štakora i skupinom štakora tretiranih s otapalom (Diab). Zabilježen je i statistički značajan porast ekspresije proBDNF, ali ne i mBDNF proteina u parijetalnom korteksu životinja. Ekspresija proteina IkBα je porasla kod skupine životinja tretiranih s rhEPO, ali dobiveni podaci nisu statistički značajni.
Abstract (english)
Diabetes mellitus is a serious, incurable, chronic disease characterised by hyperglycaemia and disorders in the metabolism of carbohydrates, fats and proteins. The incidence of this disease has increased drastically over the past decades, mostly in the elderly, but it does not bypass the younger population. Therefore, more and more research is focused on the development of new, more accessible and more efficient ways of therapeutic treatment. The effect of recombinant human erythropoietin (rhEPO) on expression and activity of SIRT1 protein (silent information regulator two protein 1), proBDNF and mBDNF protein expression as well as on IkBα protein in the pariatric cortex and/or hippocampus of rats with experimental diabetes were researched in this study. Diabetes mellitus in Hannover Wistar male rats was caused by single injection of streptozotocin (STZ). Some of the animals that developed diabetes mellitus received either rhEPO (40 µg/kg body weight) (Diab/EPO) or solvent (Diab) for four consecutive days. The control group consisted of healthy, untreated animals. The results showed that the use of rhEPO induced a statistically significant increase in SIRT1 protein expression in the brains of rats with experimental diabetes in the cortex and hippocampus compared to the control group of rats and the solvent treated group of rats (Diab). There was also a statistically significant increase in proBDNF expression, but not of mBDNF protein in the parietal cortex of animals. IkBα protein expression increased in the group of animals treated with rhEPO, but the data obtained are not statistically significant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:250552
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-09-15 09:54:19