Title UČINAK IONA METALA OTPUŠTENIH IZ ORTODONTSKIH LUKOVA NA METABOLIČKU AKTIVNOST KVASCA Saccharomyces cerevisiae Title (english) The effect of metal-ion released from different orthodontic arches of the metabolic activity of the Saccharomyces cerevisiae yeast Author Izabela Šaravanja Mentor Gordana Čanadi-Jurešić (mentor)Committee member Lara Batičić Pučar (predsjednik povjerenstva)Committee member Božena Ćurko-Cofek (član povjerenstva)Committee member Gordana Čanadi-Jurešić (član povjerenstva)Granter University of Rijeka Defense date and country 2021-09-24, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Kvasac Saccharomyces cerevisiae godinama se koristi kao modelni organizam u cijelom nizu istraživanja. Tako se koristi i za proučavanje molekularnih mehanizama oksidativnog stresa te za proučavanje učinaka metalnih iona na metaboličku aktivnost stanica. Danas postoje raznolike tehnologije i materijali koji se koriste u dentalnoj medicini. Glavne karakteristike „dobrih“ dentalnih materijala su biokompatibilnost i fizikalne kvalitete (mehanička i termička otpornost). Dentalne legure u sebi najčešće sadrže nikal, titanij, krom, molibden, kobalt i sl. Poznato je da je nikal najalergeniji metal, da je krom često povezivan s oksidativnim stresom, no i ostali metali koji se nalaze u dentalnim legurama mogu biti potencijalno toksični. Legure "rematitan" i "remanium" često se koriste u izradi dentalnih lukova zbog svoje superelastičnosti, biokompatibilnosti i lakog rukovanja prilikom postavljanja ortodontskog aparata. U ovom istraživanju proučavano je kako će stanice kvasca reagirati na ione metala koji se otpuštaju iz dentalnih lukova „rematitan“ i „remanium“ te kakva će biti njihova metabolička aktivnost ako su izloženi ovim ionima. Za određivanje metaboličke aktivnosti korišteni su: test stanične proliferacije (XTT test), „Live/Dead Yeast Viability kit“ te AO/PI boja koja se koristi za brojanje stanica na LUNA-FLTM uređaju. Krivulja rasta stanica kvasca, tretiranih i netretiranih, većeg je porasta i pravilnijeg oblika kod stanica uzgajanih na YPD hranjivoj podlozi nego kod stanica uzgajanih u umjetnoj slini. U tom slučaju stanice su imale minimalan, čak i zanemariv porast. Kod stanične proliferacije primijećeno je da promjena medija ima utjecaj na odgovor stanica obaju sojeva kvasca. Divlji soj kvasca razvije određenu toleranciju i povećava se broj stanica bez obzira što u umjetnoj slini nema hranjivih tvari poput šećera. Metabolički aktivnih stanica, određenih FUN-1 bojom, podjednako je kod oba tretmana („rematitan“ i „remanium“), dok je nešto manji udio metabolički poremećenih stanica kod tretmana „rematitan“-om. Stanice tretirane metalnim ionima mijenjaju oblik i veličinu te rastu u nakupinama („klasteri“). Metalni ioni koji su se otpuštali iz ortodontskih lukova 7 dana pokazali su najveću toksičnost.
Abstract (english) Yeast Saccharomyces cerevisiae has been used for years as a model organism in a number of studies. Thus, it is also used to study the molecular mechanisms of oxidative stress and to study the effects of metal ions on the metabolic activity of cells. Today, there are a variety of technologies and materials used in dentistry. The main characteristics to be considered in material selection are biocompatibility and quality. Dental alloys usually contain nickel, titanium, chromium, molybdenum, cobalt, etc. It is already known that nickel is the most allergenic metal, chromium is often associated with oxidative stress, but other metals found in dental alloys can as well be potentially toxic. Alloys "rematitan" and "remanium" are often used in the manufacture of dental arches due to their super-elasticity, biocompatibility and easy handling when installing an orthodontic appliance. In this study, it was studied how yeast cells will react to metal ions released from the dental arches "rematitan" and "remanium" and what will be their metabolic activity if they are exposed to these ions. To determine metabolic activity, the following were used: cell proliferation test (XTT test), „Live/Dead Yeast Viability kit“ and AOPI dye used to count cells on the LUNA-FLTM device. The growth curve of yeast cells, treated and untreated, is larger and more regular in cells grown on YPD nutrient medium than in cells grown in artificial saliva. In this case, the cells had a minimal, even negligible increase. In cell proliferation, a change in medium has been observed to affect the cell response of both strains of yeast. The wild strain of yeast develops a certain tolerance and increases the number of cells despite the fact that there are no nutrients such as sugar in artificial saliva. Metabolically active cells, determined by FUN-1 color, are the same in both treatments ("rematitan" and "remanium"), while a slightly lower proportion of metabolically disturbed cells in the treatment with "rematitan". Cells treated with metal ions change shape and size and grow in clusters ("clusters"). Metal ions released from orthodontic arches for 7 days showed the greatest toxicity.
Keywords
ortodontski lukovi
ioni metala
Saccharomyces cerevisiae
metabolička aktivnost
oksidativni stres
Keywords (english)
orthodontic arches
metal ions
Saccharomyces cerevisiae
metabolic activity
oxidative stress
Language croatian URN:NBN urn:nbn:hr:184:151860 Project Number: IPS-2020-01-7418 Study programme Title: Study of Sanitary Engineering Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2021-09-15 21:28:52
