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Scientific paper - Original scientific paper
Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays
Croatian Medical Journal, 50 (2009), 4; 370-379. urn:nbn:hr:184:948453
Braut, Tamara; Krstulja, Mira; Kujundžić, Milodar; Manestar, Dubravko; Hadžisejdić, Ita; Jonjić, Nives; Grahovac, Blaženka; Manestar, Darko

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Braut, T., Krstulja, M., Kujundžić, M., Manestar, D., Hadžisejdić, I., Jonjić, N. ... Manestar, D. (2009). Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays. Croatian Medical Journal, 50. (4), 370-379. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:948453

Braut, Tamara, et al. "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays." Croatian Medical Journal, vol. 50, no. 4, 2009, pp. 370-379. https://urn.nsk.hr/urn:nbn:hr:184:948453

Braut, Tamara, Mira Krstulja, Milodar Kujundžić, Dubravko Manestar, Ita Hadžisejdić, Nives Jonjić, Blaženka Grahovac and Darko Manestar. "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays." Croatian Medical Journal 50, no. 4 (2009): 370-379. https://urn.nsk.hr/urn:nbn:hr:184:948453

Braut, T., et al. (2009) 'Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays', Croatian Medical Journal, 50(4), pp. 370-379. Available at: https://urn.nsk.hr/urn:nbn:hr:184:948453 (Accessed 12 January 2025)

Braut T, Krstulja M, Kujundžić M, Manestar D, Hadžisejdić I, Jonjić N, and sur.. Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays. Croatian Medical Journal [Internet]. 2009 [cited 2025 January 12];50(4):370-379. Available at: https://urn.nsk.hr/urn:nbn:hr:184:948453

T. Braut, et al., "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays", Croatian Medical Journal, vol. 50, no. 4, pp. 370-379, 2009. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:948453. [Accessed: 12 January 2025]

Metadata
Title (english)Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays
AuthorTamara Braut
AuthorMira Krstulja
AuthorMilodar Kujundžić
AuthorDubravko Manestar
AuthorIta Hadžisejdić
AuthorNives Jonjić
AuthorBlaženka Grahovac
AuthorDarko Manestar
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of General Pathology and Pathological Anatomy)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Otorhinolaryngology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Otorhinolaryngology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Pathology
Abstract (english)
Aim To evaluate the importance of epidermal growth factor receptor (EGFR) protein overexpression and gene amplification in carcinogenesis of glottic cancer. Method In order to evaluate EGFR expression at protein and gene level, immunohistochemical (IHC) analysis and fluorescent in situ hybridization (FISH) were performed on tissue microarrays of laryngeal tissue (145 samples) – 38 samples of normal mucosa, 46 samples of hyperplastic lesions, and 61 samples of cancerous lesions. Results Membranous (mEGFR) and cytoplasmic (cEGFR) EGFR expression was significantly different between the analyzed groups. The differences were most striking in the suprabasal-transforming zone. IHC evaluation showed that high and low mEGFR staining contributed to the differentiation of dysplastic lesions, simple hyperplasia, and cancerous tissue, as well as between different degrees of atypia in hyperplastic lesions (P < 0.050). EGFR gene amplification was not found in simple and abnormal hyperplastic lesions, but it was confirmed in 2/21 atypical hyperplasias, indicating that gene amplification can facilitate identification of malignant potential in hyperplastic lesions. In cancerous tissue, EGFR gene amplification was found in 8/50 samples. EGFR gene amplification was found in preinvasive cancer in one patient. In invasive carcinomas, gene amplification was not associated with stage or grade. Carcinomas with gene amplification showed significantly higher cEGFR expression (basal layer P = 0.003; suprabasal layer P = 0.002). Conclusions This study confirmed an increase in EGFR protein expression and gene amplification with the increase in biological aggressiveness of glottic lesions. A correlation between EGFR gene amplification and protein expression was established. Gene amplification proved to be an early event in glottic carcinogenesis, indicating its importance for glottic cancer prevention, early detection, and protocol selection.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCroatian Medical Journal
Numberingvol. 50, no. 4, pp. 370-379
p-ISSN0353-9504
e-ISSN1332-8166
URN:NBNurn:nbn:hr:184:948453
Publication2009
Document URLhttps://hrcak.srce.hr/47857
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-01-26 10:23:12