prikaz prve stranice dokumenta Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection
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Scientific paper - Original scientific paper
Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection
Journal of neurovirology, 25 (2019), 4; 496-507. https://doi.org/10.1007/s13365-019-00750-1
Kučić, Natalia; Rački, Valentino; Jurdana, Kristina; Marcelić, Marina; Grabušić, Kristina

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Kučić, N., Rački, V., Jurdana, K., Marcelić, M. & Grabušić, K. (2019). Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection. Journal of neurovirology, 25. (4), 496-507. doi: 10.1007/s13365-019-00750-1

Kučić, Natalia, et al. "Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection." Journal of neurovirology, vol. 25, no. 4, 2019, pp. 496-507. https://doi.org/10.1007/s13365-019-00750-1

Kučić, Natalia, Valentino Rački, Kristina Jurdana, Marina Marcelić and Kristina Grabušić. "Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection." Journal of neurovirology 25, no. 4 (2019): 496-507. https://doi.org/10.1007/s13365-019-00750-1

Kučić, N., et al. (2019) 'Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection', Journal of neurovirology, 25(4), pp. 496-507. doi: 10.1007/s13365-019-00750-1

Kučić N, Rački V, Jurdana K, Marcelić M, Grabušić K. Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection. Journal of neurovirology [Internet]. 2019 [cited 2024 November 09];25(4):496-507. doi: 10.1007/s13365-019-00750-1

N. Kučić, V. Rački, K. Jurdana, M. Marcelić and K. Grabušić, "Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection", Journal of neurovirology, vol. 25, no. 4, pp. 496-507, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:610414. [Accessed: 09 November 2024]

Metadata
Title (english)Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection
AuthorNatalia Kučić
MBZ: 239812
AuthorValentino Rački
AuthorKristina Jurdana
AuthorMarina Marcelić
AuthorKristina Grabušić
MBZ: 270241
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Microglia are resident brain macrophages with key roles in development and brain homeostasis. Cytomegalovirus (CMV) readily infects microglia cells, even as a possible primary target of infection in development. Effects of CMV infection on a cellular level in microglia are still unclear ; therefore, the aim of this research was to assess the immunometabolic changes of BV-2 microglia cells following the murine cytomegalovirus (MCMV) infection. In light of that aim, we established an in vitro model of ramified BV-2 microglia (BV-2∅FCS, inducible nitric oxide synthase (iNOSlow), arginase-1 (Arg-1high), mannose receptor CD206high, and hypoxia-inducible factor 1α (HIF-1αlow)) to better replicate the in vivo conditions by removing FCS from the cultivation media, while the cells cultivated in 10% FCS DMEM displayed an ameboid morphology (BV-2FCS high, iNOShigh, Arg-1low, CD206low, and HIF- 1αhigh). Experiments were performed using both ramified and ameboid microglia, and both of them were permissive to productive viral infection. Our results indicate that MCMV significantly alters the immunometabolic phenotypic properties of BV-2 microglia cells through the manipulation of iNOS and Arg-1 expression patterns, along with an induction of a glycolytic shift in the infected cell cultures.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleJournal of neurovirology
Numberingvol. 25, no. 4, pp. 496-507
p-ISSN1355-0284
DOIhttps://doi.org/10.1007/s13365-019-00750-1
URN:NBNurn:nbn:hr:184:610414
Publication2019
ProjectNumber: 13.06.1.3.45
Title (croatian): Endocitoza MHC molekula I razreda u mikroglijalnim stanicama inficiranih citomegalovirusom
Leader: Natalia Kučić
Jurisdiction: Croatia
Funding stream: Sveučilište u Rijeci
Document URLhttp://bib.irb.hr/1016320
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2022-02-21 13:39:52