prikaz prve stranice dokumenta In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)
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Scientific paper - Original scientific paper
In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)
Journal of Essential Oil Bearing Plants, 21 (2018), 1; 77-91. https://doi.org/10.1080/0972060x.2018.1433071
Staver, Mladenka Malenica; Gobin, Ivana; Ratkaj, Ivana; Petrović, Marinko; Vulinović, Andrea; Dinarina-Sablić, Marija; Broznić, Dalibor

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Staver, M. M., Gobin, I., Ratkaj, I., Petrović, M., Vulinović, A., Dinarina Sablić, M. & Broznić, D. (2018). In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia). Journal of Essential Oil Bearing Plants, 21. (1), 77-91. doi: 10.1080/0972060x.2018.1433071

Staver, Mladenka Malenica, et al. "In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)." Journal of Essential Oil Bearing Plants, vol. 21, no. 1, 2018, pp. 77-91. https://doi.org/10.1080/0972060x.2018.1433071

Staver, Mladenka Malenica, Ivana Gobin, Ivana Ratkaj, Marinko Petrović, Andrea Vulinović, Marija Dinarina Sablić and Dalibor Broznić. "In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)." Journal of Essential Oil Bearing Plants 21, no. 1 (2018): 77-91. https://doi.org/10.1080/0972060x.2018.1433071

Staver, M. M., et al. (2018) 'In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)', Journal of Essential Oil Bearing Plants, 21(1), pp. 77-91. doi: 10.1080/0972060x.2018.1433071

Staver MM, Gobin I, Ratkaj I, Petrović M, Vulinović A, Dinarina Sablić M, and sur.. In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia). Journal of Essential Oil Bearing Plants [Internet]. 2018 [cited 2024 May 03];21(1):77-91. doi: 10.1080/0972060x.2018.1433071

M. M. Staver, et al., "In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)", Journal of Essential Oil Bearing Plants, vol. 21, no. 1, pp. 77-91, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:558420. [Accessed: 03 May 2024]

Metadata
Title (english)In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)
AuthorMladenka Malenica Staver
AuthorIvana Gobin
AuthorIvana Ratkaj
AuthorMarinko Petrović
AuthorAndrea Vulinović
AuthorMarija Dinarina-Sablić
AuthorDalibor Broznić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
In the present paper, chemical composition, in vitro antimicrobial and antiproliferative activity of the essential oil from the flowers and leaves of Helichrysum italicum (Roth) G. Don (Central Dalmatia, Croatia) as a potential replacement for standard antibiotics and chemotherapeutic agents was analyzed. Essential oil was isolated by steam distillation and analyzed by GC/MS. Antimicrobial activity was carried out by agar-well diffusion and microdilution assays with Gram (+), Gram (-) bacteria and one yeast. Antiproliferative effect, apoptosis induction and cell death on cancer cell lines: HeLa, MCF-7, SW620, CFPAC-1 and MIA PaCa-2 were analyzed by cell viability and Annexin-V assay as well as by flow cytometric analysis. In essential oil, -pinene, -curcumene and neryl acetate was found as major compounds. The antimicrobial assays, showed that essential oil had weak to moderate antimicrobial potential with S. aureus and S. epidermidis as the most sensitive bacterial strains. Essential oil treatment possessed moderate antiproliferative impact on MCF-7 and HeLa cell lines, while analyzing cell cycle treatment had no significant effect on tested cells except on MIA PaCa-2 with the highest cells increase in sub G1 phase cell cycle. However, treatment caused significantly induction of apoptosis in MCF-7 and HeLa cells, but not in MIA PaCa-2 cells. In this cell line, multiple cell death mechanism existed with involving apoptosis, senescence or necrosis. Results of the study provide a promising basis for the evaluation of the potential use of essential oil from H. italicum (Roth) G. Don as a source of alternative nature antibiotics or anticancer agents for the prevention or treatment of different diseases
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Essential Oil Bearing Plants
Numberingvol. 21, no. 1, pp. 77-91
p-ISSN0972-060X
DOIhttps://doi.org/10.1080/0972060x.2018.1433071
URN:NBNurn:nbn:hr:184:558420
Publication2018
Document URLhttp://bib.irb.hr/1049786
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2022-02-22 17:51:23