prikaz prve stranice dokumenta Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?
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Scientific paper - Original scientific paper
Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?
Environmental Monitoring and Assessment, 191 (2019), 9; 558-570. https://doi.org/10.1007/s10661-019-7733-4
Jozić, Slaven; Vukić Lušić, Darija; Aljinović, Ante; Vlakančić, Wendy; Cenov, Arijana; Vrdoljak Tomaš, Ana; Rakić, Anita; Šolić, Mladen

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Cite this document

Jozić, S., Vukić Lušić, D., Aljinović, A., Vlakančić, W., Cenov, A., Vrdoljak Tomaš, A. ... Šolić, M. (2019). Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?. Environmental Monitoring and Assessment, 191. (9), 558-570. doi: 10.1007/s10661-019-7733-4

Jozić, Slaven, et al. "Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?." Environmental Monitoring and Assessment, vol. 191, no. 9, 2019, pp. 558-570. https://doi.org/10.1007/s10661-019-7733-4

Jozić, Slaven, Darija Vukić Lušić, Ante Aljinović, Wendy Vlakančić, Arijana Cenov, Ana Vrdoljak Tomaš, Anita Rakić and Mladen Šolić. "Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?." Environmental Monitoring and Assessment 191, no. 9 (2019): 558-570. https://doi.org/10.1007/s10661-019-7733-4

Jozić, S., et al. (2019) 'Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?', Environmental Monitoring and Assessment, 191(9), pp. 558-570. doi: 10.1007/s10661-019-7733-4

Jozić S, Vukić Lušić D, Aljinović A, Vlakančić W, Cenov A, Vrdoljak Tomaš A, and sur.. Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?. Environmental Monitoring and Assessment [Internet]. 2019 [cited 2024 May 02];191(9):558-570. doi: 10.1007/s10661-019-7733-4

S. Jozić, et al., "Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?", Environmental Monitoring and Assessment, vol. 191, no. 9, pp. 558-570, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:273334. [Accessed: 02 May 2024]

Metadata
Title (english)Is TBX agar a suitable medium for monitoring Escherichia coli in bathing water using the membrane filtration method?
AuthorSlaven Jozić
MBZ: 344004
AuthorDarija Vukić Lušić
AuthorAnte Aljinović
AuthorWendy Vlakančić
AuthorArijana Cenov
AuthorAna Vrdoljak Tomaš
MBZ: 381981
AuthorAnita Rakić
MBZ: 344546
AuthorMladen Šolić
MBZ: 117375
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Environmental Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Public Health and Health Care
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Interdisciplinary Natural Sciences
Abstract (english)
The use of a suitable method for the enumeration of indicator microorganisms is of crucial importance for reliable monitoring and assessment of the quality of bathing waters. Among other characteristics, the method should be selective enough and ensure acceptable relative recovery of target microorganisms. This study presents the basic parameters, relative recovery and categorical performance characteristics of Tryptone Bile X-glucuronide (TBX) agar for Escherichia coli (E. coli) enumeration in bathing water samples using the membrane filtration method. The results of the relative recovery study, in which TBX agar was compared against temperature- modified ISO 9308- 1:2014, showed that in order to achieve a satisfactory relative recovery of E. coli with TBX agar at 44 ± 0.5 °C, the resuscitation period on a non-selective medium (Minerals Modified Glutamate Agar, MMGA) at 36 ± 2 °C is crucial. Incubation on a double-layer MMGA/TBX medium with a 6-h resuscitation period and alternating incubation on single-layer MMGA and TBX agar with a 4-h resuscitation period resulted in acceptable and very similar relative recovery. The achieved performance characteristics of the tested medium, double- layer MMGA/TBX agar, are acceptable. The selectivity was matrix-dependent and was 60.6% for inland and 69.9% for coastal waters. No significant effect of the resuscitation period on selectivity was recorded. Finally, the results showed that when the resuscitation period on a non-selective medium is included, TBX agar is a suitable medium for E. coli enumeration in bathing water samples using the membrane filtration method and that its use, theoretically, would not have negative effects on the assessment of bathing water quality
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleEnvironmental Monitoring and Assessment
Numberingvol. 191, no. 9, pp. 558-570
p-ISSN0167-6369
DOIhttps://doi.org/10.1007/s10661-019-7733-4
URN:NBNurn:nbn:hr:184:273334
Publication2019
Document URLhttp://bib.irb.hr/1015536
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2022-02-23 17:17:41