prikaz prve stranice dokumenta Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats
Access restricted to students and staff of home institution
Scientific paper - Original scientific paper
Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats
Transplantation proceedings, 50 (2018), 10; 3822-3830. https://doi.org/10.1016/j.transproceed.2018.08.035
Ćelić, Tanja; Omrčen, Hrvoje; Španjol, Josip; Bobinac, Dragica

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Ćelić, T., Omrčen, H., Španjol, J. & Bobinac, D. (2018). Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats. Transplantation proceedings, 50. (10), 3822-3830. doi: 10.1016/j.transproceed.2018.08.035

Ćelić, Tanja, et al. "Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats." Transplantation proceedings, vol. 50, no. 10, 2018, pp. 3822-3830. https://doi.org/10.1016/j.transproceed.2018.08.035

Ćelić, Tanja, Hrvoje Omrčen, Josip Španjol and Dragica Bobinac. "Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats." Transplantation proceedings 50, no. 10 (2018): 3822-3830. https://doi.org/10.1016/j.transproceed.2018.08.035

Ćelić, T., et al. (2018) 'Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats', Transplantation proceedings, 50(10), pp. 3822-3830. doi: 10.1016/j.transproceed.2018.08.035

Ćelić T, Omrčen H, Španjol J, Bobinac D. Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats. Transplantation proceedings [Internet]. 2018 [cited 2024 December 25];50(10):3822-3830. doi: 10.1016/j.transproceed.2018.08.035

T. Ćelić, H. Omrčen, J. Španjol and D. Bobinac, "Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats", Transplantation proceedings, vol. 50, no. 10, pp. 3822-3830, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:941016. [Accessed: 25 December 2024]

Metadata
Title (english)Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats
AuthorTanja Ćelić
AuthorHrvoje Omrčen
AuthorJosip Španjol
AuthorDragica Bobinac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anatomy)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Urology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Deceased donor kidneys are exposed to cold ischemic insult which makes them particularly susceptible to the effects of cold ischemic injury during hypothermic preservation resulting in high rates of delayed graft function. Bone morphogenetic protein-7 (BMP-7) is a valuable reagent in the field of tissue regeneration and preservation under ischemic conditions. Following these insights, we investigated the effect of recombinant human BMP-7 (rhBMP-7) on graft preservation during cold ischemia. The study was conducted on an experimental model of kidney cold ischemia in rats. Kidneys were perfused with University of Wisconsin (UW) saline solution, rhBMP-7, or rhBMP-7 + UW, and exposed to cold ischemia for 6, 12, and 24 hours. In tubular epithelial cells of kidneys perfused with rhBMP-7 and rhBMP-7+UW solution, the expression of BMP-7 and E-cadherin was observed after 24 hours of cold ischemia. In kidneys not perfused with rhBMP-7, high expression of transforming growth factor-beta and alpha-smooth muscle actin was found. Also, in kidneys perfused with rhBMP-7 solution, statistically higher levels of Smad1, Smad5, and Smad8 messenger RNA expressions were proven. BMP-7 maintains the morphology of kidney tissue better than UW solution during 24 hours of cold ischemia. BMP-7 prevents epithelial to mesenchymal transformation and consequently maintains epithelial phenotype of tubular cells.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleTransplantation proceedings
Numberingvol. 50, no. 10, pp. 3822-3830
p-ISSN0041-1345
DOIhttps://doi.org/10.1016/j.transproceed.2018.08.035
URN:NBNurn:nbn:hr:184:941016
Publication2018
Document URLhttp://bib.irb.hr/984391
Type of resourceText
Access conditionsInstitutional access
Terms of useLicence In copyright icon
Created on2022-02-25 13:45:02