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Scientific paper - Original scientific paper
Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins
Viruses, 14 (2022), 2; 443. https://doi.org/10.3390/v14020443
Pribanić Matešić, Marina; Kučan Brlić, Paola; Lenac Roviš, Tihana; Mačak Šafranko, Željka; Chaouat, Abigael Eva; Miklić, Karmela; Malić, Suzana; Ivanković, Nina; Schubert, Maren; Bertoglio, Federico; Markotić, Alemka; Mandelboim, Ofer; Jonjić, Stipan; Brizić, Ilija

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Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Pribanić Matešić, M., Kučan Brlić, P., Lenac Roviš, T., Mačak Šafranko, Ž., Chaouat, A. E., Miklić, K. ... Brizić, I. (2022). Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins. Viruses, 14. (2). doi: 10.3390/v14020443

Pribanić Matešić, Marina, et al. "Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins." Viruses, vol. 14, no. 2, 2022. https://doi.org/10.3390/v14020443

Pribanić Matešić, Marina, Paola Kučan Brlić, Tihana Lenac Roviš, Željka Mačak Šafranko, Abigael Eva Chaouat, Karmela Miklić, Suzana Malić, et al. "Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins." Viruses 14, no. 2 (2022). https://doi.org/10.3390/v14020443

Pribanić Matešić, M., et al. (2022) 'Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins', Viruses, 14(2). doi: 10.3390/v14020443

Pribanić Matešić M, Kučan Brlić P, Lenac Roviš T, Mačak Šafranko Ž, Chaouat AE, Miklić K, and sur.. Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins. Viruses [Internet]. 2022 February 21 [cited 2024 November 09];14(2). doi: 10.3390/v14020443

M. Pribanić Matešić, et al., "Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins", Viruses, vol. 14, no. 2, February 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:637813. [Accessed: 09 November 2024]

Metadata
Title (english)Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins
AuthorMarina Pribanić Matešić
AuthorPaola Kučan Brlić
AuthorTihana Lenac Roviš
AuthorŽeljka Mačak Šafranko
AuthorAbigael Eva Chaouat
AuthorKarmela Miklić
AuthorSuzana Malić
AuthorNina Ivanković
AuthorMaren Schubert
AuthorFederico Bertoglio
AuthorAlemka Markotić
AuthorOfer Mandelboim
AuthorStipan Jonjić
AuthorIlija Brizić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Infectious Diseases)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Infectology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
In early 2020, the COVID-19 pandemic sparked a global crisis that continues to pose a serious threat to human health and the economy. Further advancement in research is necessary and requires the availability of quality molecular tools, including monoclonal antibodies. Here, we present the development and characterization of a collection of over 40 new monoclonal antibodies directed against different SARS-CoV-2 proteins. Recombinant SARS-CoV-2 proteins were expressed, purified, and used as immunogens. Upon development of specific hybridomas, the obtained monoclonal antibody (mAb) clones were tested for binding to recombinant proteins and infected cells. We generated mAbs against structural proteins, the Spike and Nucleocapsid protein, several non-structural proteins (nsp1, nsp7, nsp8, nsp9, nsp10, nsp16) and accessory factors (ORF3a, ORF9b) applicable in flow cytometry, immunofluorescence, or Western blot. Our collection of mAbs provides a set of novel, highly specific tools that will allow a comprehensive analysis of the viral proteome, which will allow further understanding of SARS-CoV-2 pathogenesis and the design of therapeutic strategies.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleViruses
Numberingvol. 14, no. 2, 443
e-ISSN1999-4915
DOIhttps://doi.org/10.3390/v14020443
URN:NBNurn:nbn:hr:184:637813
Publication2022-02-21
ProjectNumber: IP-CORONA-2020-04-2073
Title (croatian): Dosta skrivanja CoV-2: uspostava baze monoklonskih protutijela na SARS-Cov-2
Title (english): No more hiding CoV-2: Establishment of SARS-Cov-2 mAbeom
Acronym: CoV2mAbs
Leader: Ilija Brizić
Jurisdiction: Croatia
Funding stream: IP-CORONA
ProjectNumber: KK.011.1.01.0006
Title (croatian): Jačanje kapaciteta CerVirVac-a za istraživanja u virusnoj imunologiji i vakcinologiji
Leader: Stipan Jonjić
Jurisdiction: eu
ProjectNumber: N/A
Title (croatian): Centar izvrsnosti za virusnu imunologiju i cjepiva
Title (english): Centre of Excellence for Research in Viral Immunology and the Development of New Vaccines
Acronym: CERVirVac
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: ZCI
Document URLhttps://www.mdpi.com/1999-4915/14/2/443
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-03-10 21:47:58