prikaz prve stranice dokumenta ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA
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doctoral thesis
ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA
Rijeka: University of Rijeka, 2016. urn:nbn:hr:184:386562
Tramišak-Milaković, Tamara
University of Rijeka
Faculty of Biotechnology and Drug Development

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Tramišak Milaković, T. (2016). ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA (Doctoral thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:386562

Tramišak Milaković, Tamara. "ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA." Doctoral thesis, University of Rijeka, 2016. https://urn.nsk.hr/urn:nbn:hr:184:386562

Tramišak Milaković, Tamara. "ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA." Doctoral thesis, University of Rijeka, 2016. https://urn.nsk.hr/urn:nbn:hr:184:386562

Tramišak Milaković, T. (2016). 'ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA', Doctoral thesis, University of Rijeka, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:386562

Tramišak Milaković T. ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA [Doctoral thesis]. Rijeka: University of Rijeka; 2016 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:184:386562

T. Tramišak Milaković, "ANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA", Doctoral thesis, University of Rijeka, Rijeka, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:184:386562

Metadata
TitleANTI-MÜLLEROV HORMON – POKAZATELJ KVALITETE JAJNIH STANICA I ZAMETAKA
AuthorTamara Tramišak-Milaković
MentorAnđelka Radojčić-Badovinac (mentor)
Committee memberNeda Smiljan Severinski (predsjednik povjerenstva)
Committee memberEster Pernjak-Pugel (član povjerenstva)
Committee memberAnđelka Radojčić-Badovinac (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2016-03-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Gynecology and Obstetrics
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Cilj istraživanja Istražiti da li koncentracija AMH u folikularnoj tekućini (FF AMH) može biti pokazatelj zrelosti i morfološke kvalitete zrelih MII jajnih stanica te da li je razina FF AMH povezana s uspješnošću oplodnje, morfološkom kvalitetom zigota i zametaka te ishodom postupaka. Ispitanici i metode U istraživanje su uključene 92 ispitanice liječene kontroliranom hormonskom hiperstimulacijom i mikroinjekcijom spermija (ICSI). Ispitanice su podijeljene u dvije skupine: < 35 godina (n=43) i ≥ 35 godina (n=49). Folikularna tekućina iz najvećeg folikula zasebno je aspirirana u svake ispitanice. Koncentracije AMH u serumu i folikularnoj tekućini određene su ELISA testom. Analizirana je zrelost jajnih stanica i morfološka kvaliteta zrelih MII jajnih stanica, zigota i zametaka. Rezultati Koncentracija AMH u serumu i folikularnoj tekućini bila je značajno viša u ispitanica <35 godina. Koncentracija FF AMH nije korelirala sa zrelošću jajnih stanica, morfološkom kvalitetom jajnih stanica, zigota i zametaka. Oplođene MII jajne stanice imale su značajno nižu koncentraciju FF AMH u odnosu na neoplođene jajne stanice u ispitanica <35 godina (2.56±2.0 ng/ml vs. 4.81±4.14 ng/ml, p=0.032). Zametci izabrani za prijenos imali su značajno nižu koncentraciju FF AMH u odnosu na zametke koji nisu bili odgovarajuće kvalitete za prijenos (1.73±1.26 ng/ml vs. 2.59±1.56 ng/ml, p=0.018). Zaključak Ovo istraživanje nije pokazalo korelaciju između koncentracije FF AMH i zrelosti jajnih stanica niti morfološke kvalitete zrelih MII jajnih stanica, zigota i zametaka. Međutim, nađena je značajna negativna korelacija FF AMH i uspješnosti oplodnje te kvalitete zametaka izabranih za prijenos.
Abstract (english)
Objectives The present study aimed to correlate anti-Müllerian hormone (AMH) levels in follicular fluid (FF) with oocyte maturity stages, morphological quality of metaphase II (MII) oocyte and fertilization capacity of MII oocytes. Patients and methods A total of 92 infertile women undergoing controlled ovarian stimulation and intracytoplasmic sperm injection were analyzed. Patients were divided into two groups according to age: < 35 years (n=43) and ≥ 35 years (n=49). An FF sample was obtained from a single dominant follicle in each patient for a total of 92 follicular fluid samples analyzed. AMH levels in serum and follicular fluid were measured by enzymelinked immunosorbent assay. Mature MII oocytes, zygotes, and embryos were assessed for morphological quality. Results Serum AMH levels were significantly higher in patients aged <35 years. No correlation was observed between FF AMH level and oocyte maturation stages or morphological quality of MII oocytes, zygotes and embryos. Significantly lower FF AMH levels were observed in fertilized MII oocytes than in non-fertilized MII oocytes in patients aged <35 years (2.56±2.0ng/ml vs. 4.81±4.14ng/ml, p=0.032). Also, significantly lower FF AMH levels were observed in embryos selected for transfer than in non-selected embryos (1.73±1.26 ng/ml vs. 2.59±1.56 ng/ml, p=0.018). Conclusion The present study revealed no correlation between FF AMH and oocyte maturity stage or morphological quality of MII oocyte, zygote and embryos. However, significant negative correlation between FF AMH and fertilization capacity of MII oocytes and quality of embryos selected for transfer were found
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:386562
Study programmeTitle: Medicinal chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, interdisciplinarna područja znanosti, polje biotehnologija u biomedicini (doktor/doktorica znanosti, interdisciplinarna područja znanosti, polje biotehnologija u biomedicini)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2022-03-18 15:35:15