prikaz prve stranice dokumenta Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
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Scientific paper - Original scientific paper
Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
Viruses, 13 (2021), 12; 2413. https://doi.org/10.3390/v13122413
Pavletić, Martina; Mazor, Marija; Lerga, Mate; Mileta, Tatjana; Železnjak, Jelena; Ružić, Tina; Ravlić, Sanda; Palčevski, Dora; Kirinčić, Jelena; Mežnarić, Silvestar; Mišković, Ana; Materljan, Maja; Šustić, Alan; Lisnić, Berislav; Juranić Lisnić, Vanda More authors...

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Pavletić, M., Mazor, M., Lerga, M., Mileta, T., Železnjak, J., Ružić, T. ... Juranić Lisnić, V. (2021). Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments. Viruses, 13. (12). doi: 10.3390/v13122413

Pavletić, Martina, et al. "Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments." Viruses, vol. 13, no. 12, 2021. https://doi.org/10.3390/v13122413

Pavletić, Martina, Marija Mazor, Mate Lerga, Tatjana Mileta, Jelena Železnjak, Tina Ružić, Sanda Ravlić, et al. "Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments." Viruses 13, no. 12 (2021). https://doi.org/10.3390/v13122413

Pavletić, M., et al. (2021) 'Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments', Viruses, 13(12). doi: 10.3390/v13122413

Pavletić M, Mazor M, Lerga M, Mileta T, Železnjak J, Ružić T, and sur.. Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments. Viruses [Internet]. 2021 December 02 [cited 2024 April 29];13(12). doi: 10.3390/v13122413

M. Pavletić, et al., "Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments", Viruses, vol. 13, no. 12, December 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:892584. [Accessed: 29 April 2024]

Metadata
Title (english)Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
AuthorMartina Pavletić
AuthorMarija Mazor
AuthorMate Lerga
AuthorTatjana Mileta
AuthorJelena Železnjak
AuthorTina Ružić
AuthorSanda Ravlić
AuthorDora Palčevski
AuthorJelena Kirinčić
AuthorSilvestar Mežnarić
AuthorAna Mišković
AuthorMaja Materljan
AuthorAlan Šustić
AuthorBerislav Lisnić
AuthorVanda Juranić Lisnić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anaesthesiology,reanimatology and Intensive Care)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleViruses
Numberingvol. 13, no. 12, 2413
e-ISSN1999-4915
DOIhttps://doi.org/10.3390/v13122413
URN:NBNurn:nbn:hr:184:892584
Publication2021-12-02
ProjectNumber: KK.011.1.01.0006
Title (croatian): Jačanje kapaciteta CerVirVac-a za istraživanja u virusnoj imunologiji i vakcinologiji
Leader: Stipan Jonjić
Jurisdiction: eu
ProjectNumber: N/A
Title (croatian): Centar izvrsnosti za virusnu imunologiju i cjepiva
Title (english): Centre of Excellence for Research in Viral Immunology and the Development of New Vaccines
Acronym: CERVirVac
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: ZCI
Document URLhttps://www.mdpi.com/1999-4915/13/12/2413
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-04-01 12:25:33