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Scientific paper - Original scientific paper
Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences
Genes, 12 (2021), 12; 1999. https://doi.org/10.3390/genes12121999
Zenic, Lucija; Polančec, Denis; Hudetz, Damir; Jeleč, Željko; Rod, Eduard; Vidović, Dinko; Starešinić, Mario; Sabalic, Srećko; Vrdoljak, Trpimir; Petrović, Tadija; Cukelj, Fabijan; Molnar, Vilim; Cemerin, Martin; Matišić, Vid; Brlek, Petar; Djukić Koroljević, Zrinka; Borić, Igor; Lauc, Gordan; Primorac, Dragan More authors...

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Zenic, L., Polančec, D., Hudetz, D., Jeleč, Ž., Rod, E., Vidović, D. ... Primorac, D. (2021). Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences. Genes, 12. (12), 1. doi: 10.3390/genes12121999

Zenic, Lucija, et al. "Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences." Genes, vol. 12, no. 12, 2021, pp. 1. https://doi.org/10.3390/genes12121999

Zenic, Lucija, Denis Polančec, Damir Hudetz, Željko Jeleč, Eduard Rod, Dinko Vidović, Mario Starešinić, et al. "Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences." Genes 12, no. 12 (2021): 1. https://doi.org/10.3390/genes12121999

Zenic, L., et al. (2021) 'Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences', Genes, 12(12), pp. 1. doi: 10.3390/genes12121999

Zenic L, Polančec D, Hudetz D, Jeleč Ž, Rod E, Vidović D, and sur.. Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences. Genes [Internet]. 2021 December 16 [cited 2024 November 04];12(12):1. doi: 10.3390/genes12121999

L. Zenic, et al., "Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences", Genes, vol. 12, no. 12, pp. 1, December 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:793998. [Accessed: 04 November 2024]

Metadata
Title (english)Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences
AuthorLucija Zenic
AuthorDenis Polančec
AuthorDamir Hudetz
AuthorŽeljko Jeleč
AuthorEduard Rod
AuthorDinko Vidović
AuthorMario Starešinić
AuthorSrećko Sabalic
AuthorTrpimir Vrdoljak
AuthorTadija Petrović
AuthorFabijan Cukelj
AuthorVilim Molnar
AuthorMartin Cemerin
AuthorVid Matišić
AuthorPetar Brlek
AuthorZrinka Djukić Koroljević
AuthorIgor Borić
AuthorGordan Lauc
AuthorDragan Primorac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Biology and Medical Genetics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Orthopedics
Abstract (english)
Mesenchymal stem/stromal cells or medicinal signaling cells (MSC)-based therapy holds promise as a beneficial strategy for treating knee OA (osteoarthritis), but there is no standardized protocols nor mechanistic understanding. In order to gain a better insight into the human MSC from adipose tissue applied for autologous OA treatment, we performed extensive comparative immunophenotyping of the stromal vascular fraction from lipoaspirate or microfragmented lipoaspirates by polychromatic flow cytometry and investigated the cellular components considered responsible for cartilage regeneration. We found an enrichment of the regenerative cellular niche of the clinically applied microfragmented stromal vascular fraction. Sex-related differences were observed in the MSC marker expression and the ratio of the progenitor cells from fresh lipoaspirate, which, in female patients, contained a higher expression of CD90 on the three progenitor cell types including pericytes, a higher expression of CD105 and CD146 on CD31highCD34high endothelial progenitors as well as of CD73 on supra-adventitialadipose stromal cells. Some of these MSC-expression differences were present after microfragmentation and indicated a differential phenotype pattern of the applied MSC mixture in female and male patients. Our results provide a better insight into the heterogeneity of the adipose MSC subpopulations serving as OA therapeutics, with an emphasis on interesting differences between women and men.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleGenes
Numberingvol. 12, no. 12, p. 1, 1999
e-ISSN2073-4425
DOIhttps://doi.org/10.3390/genes12121999
URN:NBNurn:nbn:hr:184:793998
Publication2021-12-16
Document URLhttps://www.mdpi.com/2073-4425/12/12/1999
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-04-07 14:03:13