prikaz prve stranice dokumenta The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase
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Scientific paper - Original scientific paper
The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase
Journal of Virology, 70 (1996), 9; 6340-6346. urn:nbn:hr:184:830706
Michel, Detlef; Pavić, Ivica; Zimmermann, Albert; Haupt, Edith; Wunderlich, Kirsten; Heuschmid, Maria; Mertens, Thomas

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Michel, D., Pavić, I., Zimmermann, A., Haupt, E., Wunderlich, K., Heuschmid, M. & Mertens, T. (1996). The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase. Journal of Virology, 70. (9), 6340-6346. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:830706

Michel, Detlef, et al. "The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase." Journal of Virology, vol. 70, no. 9, 1996, pp. 6340-6346. https://urn.nsk.hr/urn:nbn:hr:184:830706

Michel, Detlef, Ivica Pavić, Albert Zimmermann, Edith Haupt, Kirsten Wunderlich, Maria Heuschmid and Thomas Mertens. "The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase." Journal of Virology 70, no. 9 (1996): 6340-6346. https://urn.nsk.hr/urn:nbn:hr:184:830706

Michel, D., et al. (1996) 'The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase', Journal of Virology, 70(9), pp. 6340-6346. Available at: https://urn.nsk.hr/urn:nbn:hr:184:830706 (Accessed 25 December 2024)

Michel D, Pavić I, Zimmermann A, Haupt E, Wunderlich K, Heuschmid M, and sur.. The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase. Journal of Virology [Internet]. 1996 [cited 2024 December 25];70(9):6340-6346. Available at: https://urn.nsk.hr/urn:nbn:hr:184:830706

D. Michel, et al., "The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase", Journal of Virology, vol. 70, no. 9, pp. 6340-6346, 1996. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:830706. [Accessed: 25 December 2024]

Metadata
Title (english)The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase
AuthorDetlef Michel
AuthorIvica Pavić
AuthorAlbert Zimmermann
AuthorEdith Haupt
AuthorKirsten Wunderlich
AuthorMaria Heuschmid
AuthorThomas Mertens
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Infectious Diseases)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The temporal expression of the UL97 gene product during human cytomegalovirus (HCMV) infection of human foreskin fibroblasts (HFF) and subcellular localization of this protein were analyzed by using a polyclonal antiserum raised against a truncated UL97 protein of 47 kDa. The UL97 protein was detectable 16 h after infection by Western blot (immunoblot) analysis. Since only reduced UL97 expression occurred in the presence of two inhibitors of DNA replication, phosphonoacetic acid and ganciclovir, we conclude that UL97 is an early-late gene, requiring DNA replication for maximum expression. By indirect immunofluorescence, the protein could be visualized in the nuclei of virus-infected HFF 22 h after infection. Nuclear localization of the UL97 protein was also detected in thymidine kinase-deficient 143B cells infected with a recombinant vaccinia virus containing the entire UL97 open reading frame (ORF), as well as in HFF transiently expressing the entire UL97 ORF under the control of HCMV major immediate-early promoter. However, transiently expressed 5'-terminal deletion mutants of the UL97 ORF in addition showed a cytoplasmic localization of the UL97 protein, confirming the presence of a nuclear localization site in the N-terminal region of the protein. Our high-pressure liquid chromatography analyses confirmed the ganciclovir phosphorylation by the UL97 protein, but no specific phosphorylation of natural nucleosides was observed, indicating that the UL97 protein is not a nucleoside kinase. During plaque purification of recombinant UL97-deficient HCMV, this virus was growth defective ; hence, we presume that UL97 may be essential for the viral life cycle.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Virology
Numberingvol. 70, no. 9, pp. 6340-6346
p-ISSN0022-538X
URN:NBNurn:nbn:hr:184:830706
Publication1996
Document URLhttp://bib.irb.hr/811812
Type of resourceText
Access conditionsOpen access
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Created on2022-04-20 14:11:36