prikaz prve stranice dokumenta Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
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Scientific paper - Original scientific paper
Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
Biomedicines, 10 (2022), 4; 785. https://doi.org/10.3390/biomedicines10040785
Krušić Alić, Vedrana; Malenica, Mladenka; Biberić, Maša; Zrna, Siniša; Valenčić, Lara; Šuput, Aleksandar; Kalagac Fabris, Lada; Wechtersbach, Karmen; Kojc, Nika; Kurtjak, Mario; Kučić, Natalia; Grabušić, Kristina

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Krušić Alić, V., Malenica, M., Biberić, M., Zrna, S., Valenčić, L., Šuput, A. ... Grabušić, K. (2022). Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods. Biomedicines, 10. (4). doi: 10.3390/biomedicines10040785

Krušić Alić, Vedrana, et al. "Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods." Biomedicines, vol. 10, no. 4, 2022. https://doi.org/10.3390/biomedicines10040785

Krušić Alić, Vedrana, Mladenka Malenica, Maša Biberić, Siniša Zrna, Lara Valenčić, Aleksandar Šuput, Lada Kalagac Fabris, et al. "Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods." Biomedicines 10, no. 4 (2022). https://doi.org/10.3390/biomedicines10040785

Krušić Alić, V., et al. (2022) 'Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods', Biomedicines, 10(4). doi: 10.3390/biomedicines10040785

Krušić Alić V, Malenica M, Biberić M, Zrna S, Valenčić L, Šuput A, and sur.. Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods. Biomedicines [Internet]. 2022 March 27 [cited 2024 July 05];10(4). doi: 10.3390/biomedicines10040785

V. Krušić Alić, et al., "Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods", Biomedicines, vol. 10, no. 4, March 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:277557. [Accessed: 05 July 2024]

Metadata
Title (english)Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
AuthorVedrana Krušić Alić
AuthorMladenka Malenica
AuthorMaša Biberić
AuthorSiniša Zrna
AuthorLara Valenčić
AuthorAleksandar Šuput
AuthorLada Kalagac Fabris
AuthorKarmen Wechtersbach
AuthorNika Kojc
AuthorMario Kurtjak
AuthorNatalia Kučić
MBZ: 239812
AuthorKristina Grabušić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Extracellular vesicles (EVs) are a versatile group of cell-secreted membranous nanoparticles present in body fluids. They have an exceptional diagnostic potential due to their molecular content matching the originating cells and accessibility from body fluids. However, methods for EV isolation are still in development, with size exclusion chromatography (SEC) emerging as a preferred method. Here we compared four types of SEC to isolate EVs from the CSF of patients with severe traumatic brain injury. A pool of nine CSF samples was separated by SEC columns packed with Sepharose CL-6B, Sephacryl S-400 or Superose 6PG and a ready-to-use qEV10/70 nm column. A total of 46 fractions were collected and analysed by slot-blot followed by Ponceau staining. Immunodetection was performed for albumin, EV markers CD9, CD81, and lipoprotein markers ApoE and ApoAI. The size and concentration of nanoparticles in fractions were determined by tunable resistive pulse sensing and EVs were visualised by transmission electron microscopy. We show that all four SEC techniques enabled separation of CSF into nanoparticle- and free protein-enriched fractions. Sepharose CL-6B resulted in a significantly higher number of separated EVs while lipoproteins were eluted together with free proteins. Our data indicate that Sepharose CL-6B is suitable for isolation of EVs from CSF and their separation from lipoproteins.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBiomedicines
Numberingvol. 10, no. 4, 785
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines10040785
URN:NBNurn:nbn:hr:184:277557
Publication2022-03-27
Document URLhttps://www.mdpi.com/2227-9059/10/4/785
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-04-26 11:53:25