prikaz prve stranice dokumenta Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection
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Scientific paper - Original scientific paper
Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection
Life (Basel), 11 (2021), 8; 867. https://doi.org/10.3390/life11080867
Pavišić, Valentino; Mahmutefendić Lučin, Hana; Blagojević Zagorac, Gordana; Lučin, Pero

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Pavišić, V., Mahmutefendić Lučin, H., Blagojević Zagorac, G. & Lučin, P. (2021). Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection. Life (Basel), 11. (8). doi: 10.3390/life11080867

Pavišić, Valentino, et al. "Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection." Life (Basel), vol. 11, no. 8, 2021. https://doi.org/10.3390/life11080867

Pavišić, Valentino, Hana Mahmutefendić Lučin, Gordana Blagojević Zagorac and Pero Lučin. "Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection." Life (Basel) 11, no. 8 (2021). https://doi.org/10.3390/life11080867

Pavišić, V., et al. (2021) 'Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection', Life (Basel), 11(8). doi: 10.3390/life11080867

Pavišić V, Mahmutefendić Lučin H, Blagojević Zagorac G, Lučin P. Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection. Life (Basel) [Internet]. 2021 August 23 [cited 2024 May 02];11(8). doi: 10.3390/life11080867

V. Pavišić, H. Mahmutefendić Lučin, G. Blagojević Zagorac and P. Lučin, "Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection", Life (Basel), vol. 11, no. 8, August 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:236112. [Accessed: 02 May 2024]

Metadata
Title (english)Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection
AuthorValentino Pavišić
AuthorHana Mahmutefendić Lučin
AuthorGordana Blagojević Zagorac
AuthorPero Lučin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Abstract (english)
Shortly after entering the cells, cytomegaloviruses (CMVs) initiate massive reorganization of cellular endocytic and secretory pathways, which results in the forming of the cytoplasmic virion assembly compartment (AC). We have previously shown that the formation of AC in murine CMV- (MCMV) infected cells begins in the early phase of infection (at 4-6 hpi) with the pre-AC establishment. Pre-AC comprises membranes derived from the endosomal recycling compartment, early endosomes, and the trans-Golgi network, which is surrounded by fragmented Golgi cisterns. To explore the importance of Arf GTPases in the biogenesis of the pre-AC, we infected Balb 3T3 cells with MCMV and analyzed the expression and intracellular localization of Arf proteins in the early phases (up to 16 hpi) of infection and the development of pre-AC in cells with a knockdown of Arf protein expression by small interfering RNAs (siRNAs). Herein, we show that even in the early phase, MCMVs cause massive reorganization of the Arf system of the host cells and induce the over-recruitment of Arf proteins onto the membranes of pre-AC. Knockdown of Arf1, Arf3, Arf4, or Arf6 impaired the establishment of pre-AC. However, the knockdown of Arf1 and Arf6 also abolished the establishment of infection. Our study demonstrates that Arf GTPases are required for different steps of early cytomegalovirus infection, including the establishment of the pre-AC.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleLife (Basel)
Numberingvol. 11, no. 8, 867
BPC1800 CHF
e-ISSN0000-0000
DOIhttps://doi.org/10.3390/life11080867
URN:NBNurn:nbn:hr:184:236112
Online first2021-08-23
ProjectNumber: IP-2020-02-1323
Title (croatian): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Acronym: Arf & CMV
Leader: Gordana Blagojević Zagorac
Jurisdiction: Croatia
Document URLhttps://www.mdpi.com/2075-1729/11/8/867
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-ShareAlike 3.0 Unported (CC BY-SA 3.0) icon
Created on2022-05-18 06:32:30