prikaz prve stranice dokumenta Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro
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Scientific paper - Original scientific paper
Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro
International Journal of Environmental Research and Public Health, 19 (2022), 10; 6177. https://doi.org/10.3390/ijerph19106177
Piletić, Kaća; Kovač, Bruno; Perčić, Marko; Žigon, Jure; Broznić, Dalibor; Karleuša, Ljerka; Lučić Blagojević, Sanja; Oder, Martina; Gobin, Ivana

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Piletić, K., Kovač, B., Perčić, M., Žigon, J., Broznić, D., Karleuša, Lj. ... Gobin, I. (2022). Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro. International Journal of Environmental Research and Public Health, 19. (10). doi: 10.3390/ijerph19106177

Piletić, Kaća, et al. "Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro." International Journal of Environmental Research and Public Health, vol. 19, no. 10, 2022. https://doi.org/10.3390/ijerph19106177

Piletić, Kaća, Bruno Kovač, Marko Perčić, Jure Žigon, Dalibor Broznić, Ljerka Karleuša, Sanja Lučić Blagojević, Martina Oder and Ivana Gobin. "Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro." International Journal of Environmental Research and Public Health 19, no. 10 (2022). https://doi.org/10.3390/ijerph19106177

Piletić, K., et al. (2022) 'Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro', International Journal of Environmental Research and Public Health, 19(10). doi: 10.3390/ijerph19106177

Piletić K, Kovač B, Perčić M, Žigon J, Broznić D, Karleuša Lj, and sur.. Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro. International Journal of Environmental Research and Public Health [Internet]. 2022 [cited 2024 December 25];19(10). doi: 10.3390/ijerph19106177

K. Piletić, et al., "Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro", International Journal of Environmental Research and Public Health, vol. 19, no. 10, 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:346825. [Accessed: 25 December 2024]

Metadata
Title (english)Disinfecting Action of Gaseous Ozone on OXA-48- Producing Klebsiella pneumoniae Biofilm In Vitro
AuthorKaća Piletić
AuthorBruno Kovač
AuthorMarko Perčić
MBZ: 353085
AuthorJure Žigon
AuthorDalibor Broznić
MBZ: 270072
AuthorLjerka Karleuša
MBZ: 334545
AuthorSanja Lučić Blagojević
MBZ: 186655
AuthorMartina Oder
AuthorIvana Gobin
Author's institutionUniversity of Rijeka
Faculty of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Klebsiella pneumoniae is an emerging multidrug- resistant pathogen that can contaminate hospital surfaces in the form of a biofilm which is hard to remove with standard disinfectants. Because of biofilm resistance to conservative disinfectants, the application of new disinfection technologies is becoming more frequent. Ozone gas has antimicrobial activity but there is lack of data on its action against K. pneumoniae biofilm. The aim of this study was to investigate the effects and mechanisms of action of gaseous ozone on the OXA-48-procuding K. pneumoniae biofilm. A 24 h biofilm of K. pneumoniae formed on ceramic tiles was subsequently exposed to different concentrations of ozone during one and two hours to determine the optimal ozone concentration. Afterwards, the total bacteria count, total biomass and oxidative stress levels were monitored. A total of 25 ppm of gaseous ozone was determined to be optimal ozone concentration and caused reduction in total bacteria number in all strains of K. pneumoniae for 2.0 log10 CFU/cm2, followed by reduction in total biomass up to 88.15%. Reactive oxygen species levels significantly increased after the ozone treatment at 182% for the representative K. pneumoniae NCTC 13442 strain. Ozone gas in the concentration of 25 ppm caused significant biofilm reduction but did not completely eradicate the K. pneumoniae biofilm formed on ceramics. In conclusion, ozone gas has great potential to be used as an additional hygiene measure in joint combat against biofilm in hospital environments.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Environmental Research and Public Health
Numberingvol. 19, no. 10, 6177
p-ISSN1660-4601
DOIhttps://doi.org/10.3390/ijerph19106177
URN:NBNurn:nbn:hr:184:346825
Publication2022
Document URLhttp://bib.irb.hr/1197491
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-06-29 09:38:01