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doctoral thesis
IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE
Rijeka: University of Rijeka, Faculty of Medicine, 2022. urn:nbn:hr:184:156853
Zulijani, Ana
University of Rijeka
Faculty of Medicine
Department of Physiology, Immunology and Pathophxsiology
University of Rijeka

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Zulijani, A. (2022). IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE (Doctoral thesis). Rijeka: University of Rijeka, Faculty of Medicine; Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:156853

Zulijani, Ana. "IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE." Doctoral thesis, University of Rijeka, Faculty of Medicine; University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:184:156853

Zulijani, Ana. "IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE." Doctoral thesis, University of Rijeka, Faculty of Medicine; University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:184:156853

Zulijani, A. (2022). 'IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE', Doctoral thesis, University of Rijeka, Faculty of Medicine; University of Rijeka, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:184:156853

Zulijani A. IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE [Doctoral thesis]. Rijeka: University of Rijeka, Faculty of Medicine; Rijeka: University of Rijeka; 2022 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:184:156853

A. Zulijani, "IZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE", Doctoral thesis, University of Rijeka, Faculty of Medicine, Rijeka; University of Rijeka, Rijeka, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:156853

Metadata
TitleIZRAŽAJ METALOTIONEINA I MEGALINA U PREMALIGNIM I MALIGNIM EPITELNIM LEZIJAMA SLUZNICE USNE ŠUPLJINE
Title (english)METALLOTHIONEIN AND MEGALIN EXSPRESSION IN PREMALIGNANT AND MALIGNANT EPITHELIAL LESION OF THE ORAL MUCOSA
AuthorAna Zulijani
MentorHrvoje Jakovac (mentor)
MentorTomislav Ćabov (komentor)
Committee memberMargita Belušić-Gobić (predsjednik povjerenstva)
Committee memberBerislav Perić (član povjerenstva)
Committee memberZlatko Trobonjača (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Rijeka
GranterUniversity of Rijeka
Rijeka
Defense date and country2022, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Universal decimal classification
(UDC)		616.31 - Stomatology
Abstract
Cilj: Cilj ovog rada bio je procijeniti izražaj, tkivnu raspodjelu, odnos i moguće interakcije između metalotioneina (MT) i megalina (LRP-2) u različitim patohistološkim stupnjevima karcinoma pločastih stanica usne šupljine (OSCC) i potencijalno malignim oralnim poremećajima (PMOP- oralna leukoplakija (OL) i oralni lihen planus (OLP)), te procijeniti njihov odnos prema proliferacijskim biljezima i biljezima apoptoze. Materijali i metode: Ova retrospektivna studija uključivala je arhivirane uzorke tkiva 122 pacijenata i kontrolnih ispitanika. Izražaj proteina ispitan je imunohistokemijom i imunofluorescencijom, a kvantifikacija obojenja procijenjena je korištenjem Image J softvera. Interakcija proteina u tumorskom tkivu testirana je i vizualizirana PLA metodom (prema engl. Proximity Ligation Assay). Mann-Whitney i Kruskal-Wallis testovi korišteni su za utvrđivanje značaja razlike između svake skupine, dok je Pearsonov koeficijent korelacije proveden kako bi se testirala korelacija. Rezultati: Izražaj oba proteina značajno se razlikovao između svake skupine koja pokazuje isti obrazac postupnog povećanja od oralnog lihen planusa do slabo diferenciranog OSCC-a. Štoviše, utvrđeno je da MT i megalin suizražavaju i međusobno djeluju u tumorskom tkivu, a njihov izražaj pozitivno korelira unutar cjelokupne ispitivane skupine. Nalazi istaknutog izražaja megalin a u jezgri i kromosomima sugeriraju da prolazi reguliranu intramembransku proteolizu (RIP) nakon vezanja s MT-a, što ukazuje na njegovu sposobnost izravnog utjecaja na genski izražaj i staničnu diobu u tumorskom tkivu. Zaključak: Dobiveni podaci ukazuju na potencijalnu interakciju MT-megalina u onkogenezi.
Abstract (english)
Objectives: The aim of this study was to assess the expression, tissue distribution, relationship and possible interactions between metallothioneins (MTs) and megalin (LRP-2) in different grades of oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD, oral leukoplakia (Ol) and oral lichen planus (OLP)), as well as to determine their relationship to proliferative markers and markers of apoptosis. Material and Methods: This retrospective study included archived samples of 114 patients and control subjects. Protein expression was examined by immunohistochemistry and immunofluorescence, and staining quantification was performed by ImageJ software. Protein interaction in cancer tissue was tested and visualized by proximity ligation assay. MannWhitney and Kruskal-Wallis tests were used to determine the significance of differences between each group, whereas Pearson correlation coefficient was performed to test correlation. Results: Expression of both proteins differed significantly between each group showing the same pattern of gradual increasing from oral lichen planus to poorly differentiated OSCC. Moreover, MTs and megalin were found to co-express and interact in cancer tissue, and their expression positively correlated within the overall study group. Findings of prominent nuclear and chromosomal megalin expression suggest that it undergoes regulated intramembrane proteolysis upon MTs binding, indicating its ability to directly affect gene expression and cellular division in cancer tissue. Conclusion: Data obtained point to the onco-driving potential of MTs-megalin interaction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:156853
Promotion2022
Study programmeTitle: Biomedicine Postgraduate (doctoral) study programme
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2022-08-03 08:19:51