| Abstract | Zrak je važan fizikalno-ekološki faktor okoliša neophodan za život svih živih bića, te kao takav
direktno utječe na zdravlje i kvalitetu života. S obzirom da je većina onečišćenja zraka antropogena
podrijetla, kvaliteta zraka prvenstveno ovisi o čovjeku koji ga je dužan zaštititi. Iako postoje
različite vrste onečišćenja, u ovom istraživanju ispituje se mikrobiološka kvaliteta zraka. Tako je
u ovom radu ispitana mikrobiološka kvaliteta zraka u zatvorenim laboratorijskim prostorijama
Mikrobiološkog odjela Nastavnog zavoda za javno zdravstvo Primorsko-goranske županije.
Uzorkovanje zraka je provedeno u Laboratoriju za dijagnostiku respiratornih i sustavnih infekcija,
te na šalteru Mikrobiološkog odjela u prizemlju ustanove. U Laboratoriju za dijagnostiku
respiratornih i sustavnih infekcija provedena je i dezinfekcija nakon uzorkovanja zraka, nakon
čega se zrak ponovno uzorkovao kako bi se utvrdila njezina uspješnost. Zrak je uzorkovan 28.
travnja 2022. godine u Laboratoriju za dijagnostiku respiratornih i sustavnih infekcija, te 29.
travnja 2022. godine na šalteru Mikrobiološkog odjela. Zrak se uzorkovao uređajem MAS-100, a
za dezinfekciju se koristio uređaj SpeedyCare UV. Za potrebe istraživanja korištene su 4 različite
hranjive podloge: TSA agar, UriSelect agar, kromogeni agar, te Sabouraud agar. Nakon što su
porasle kolonije, one su prebrojane, te je nakon toga uslijedila daljnja identifikacija
mikroorganizama. Rezultati istraživanja su pokazali kako je manji broj mikroorganizama prisutno
u prostorijama Laboratorija za dijagnostiku respiratornih i sustavnih infekcija u odnosu na šalter
Mikrobiološkog odjela. Također, gotovo sve bakterijske vrste koje su identificirane ne
predstavljaju opasnost po zdravlje čovjeka s obzirom da je većina njih normalno prisutna na epitelu
kože ili sluznici nosa. Dezinfekcijom zraka smanjio se broj bakterijskih kolonija u prostoriji, što
je potvrdilo učinkovitost uređaja za dezinfekciju, ali nije utjecao na smanjenje broja kvasaca i
plijesni što je ukazalo na to da uređaj za dezinfekciju nije sredstvo namijenjeno za suzbijanje te
vrste mikroorganizama. |
| Abstract (english) | Air is an important physical-ecological environmental factor necessary for the life of all living
beings, and as such, it directly affects health and quality of life. Given that the majority of air
pollution is of anthropogenic origin, air quality primarily depends on man, who is obliged to
protect it. Although there are different types of pollution, this research examines the
microbiological quality of the air. In this research it was examined the microbiological quality of
the air in closed laboratory rooms of the Microbiology Department of the Teaching Institute of
Public Health of Primorje-Gorski Kotar County. Air sampling was carried out in the Laboratory
for the diagnosis of respiratory and systemic infections, and at the counter of the Microbiology
Department on the ground floor of the institution. In the Laboratory for the diagnosis of respiratory
and systemic infections, disinfection was carried out after air sampling, after which the air was
resampled to determine its success. The air was sampled on April 28, 2022 in the Laboratory for
Diagnostics of Respiratory and Systemic Infections, and on April 29, 2022 at the counter of the
Microbiology Department. The air was sampled with the MAS-100 device, and the SpeedyCare
UV device was used for disinfection. For research purposes, 4 different nutrient media were used:
TSA agar, UriSelect agar, chromogenic agar, and Sabouraud agar. After the colonies had grown,
they were counted, followed by further identification of the microorganisms. The results of the
research showed that a smaller number of microorganisms are present in the premises of the
Laboratory for the diagnosis of respiratory and systemic infections compared to the counter of the
Microbiology Department. Also, almost all bacterial species that have been identified do not pose
a threat to human health, considering that most of them are normally present on the epithelium of
the skin or the mucous membrane of the nose. Disinfection of the air reduced the number of
bacterial colonies in the room, which confirmed the effectiveness of the disinfection device, but
did not affect the reduction of the number of yeasts and molds, which indicated that the disinfection
device is not a means intended to suppress this type of microorganisms. |
