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Scientific paper - Original scientific paper
SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study
Viruses, 14 (2022), 9; 1966. https://doi.org/10.3390/v14091966
Brlić, Paola Kučan; Pavletić, Martina; Lerga, Mate; Krstanović, Fran; Matešić, Marina Pribanić; Miklić, Karmela; Malić, Suzana; Mikša, Leonarda; Pajcur, Maja; Peruč, Dolores; Schubert, Maren; Bertoglio, Federico; Arapović, Jurica; Protić, Alen; Šustić, Alan; Milošević, Marko; Šain, Luka Čičin; Jonjić, Stipan; Lisnić, Vanda Juranić; Brizić, Ilija More authors...

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Brlić, P. K., Pavletić, M., Lerga, M., Krstanović, F., Matešić, M. P., Miklić, K. ... Brizić, I. (2022). SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study. Viruses, 14. (9). doi: 10.3390/v14091966

Brlić, Paola Kučan, et al. "SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study." Viruses, vol. 14, no. 9, 2022. https://doi.org/10.3390/v14091966

Brlić, Paola Kučan, Martina Pavletić, Mate Lerga, Fran Krstanović, Marina Pribanić Matešić, Karmela Miklić, Suzana Malić, et al. "SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study." Viruses 14, no. 9 (2022). https://doi.org/10.3390/v14091966

Brlić, P. K., et al. (2022) 'SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study', Viruses, 14(9). doi: 10.3390/v14091966

Brlić PK, Pavletić M, Lerga M, Krstanović F, Matešić MP, Miklić K, and sur.. SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study. Viruses [Internet]. 2022 September 04 [cited 2024 December 24];14(9). doi: 10.3390/v14091966

P. K. Brlić, et al., "SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study", Viruses, vol. 14, no. 9, September 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:927357. [Accessed: 24 December 2024]

Metadata
Title (english)SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study
AuthorPaola Kučan Brlić
AuthorMartina Pavletić
AuthorMate Lerga
AuthorFran Krstanović
AuthorMarina Pribanić Matešić
AuthorKarmela Miklić
AuthorSuzana Malić
AuthorLeonarda Mikša
AuthorMaja Pajcur
AuthorDolores Peruč
AuthorMaren Schubert
AuthorFederico Bertoglio
AuthorJurica Arapović
AuthorAlen Protić
AuthorAlan Šustić
AuthorMarko Milošević
AuthorLuka Čičin Šain
AuthorStipan Jonjić
AuthorVanda Juranić Lisnić
AuthorIlija Brizić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anaesthesiology,reanimatology and Intensive Care)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Infectology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract (english)
Studies assessing the dynamics and duration of antibody responses following SARS-CoV-2 infection or vaccination are an invaluable tool for vaccination schedule planning, assessment of risk groups and management of pandemics. In this study, we developed and employed ELISA assays to analyze the humoral responses to Nucleocapsid and Spike proteins in vaccinated health-care workers (HCW) and critically ill COVID-19 patients. Sera of more than 1000 HCWs and critically ill patients from the Clinical Hospital Center Rijeka were tested across a one-year period, encompassing the spread of major SARS-CoV-2 variants of concern (VOCs). We observed 97% of seroconversion in HCW cohort as well as sustained anti-Spike antibody response in vaccinees for more than 6 months. In contrast, the infection-induced anti-Nucleocapsid response was waning significantly in a six-month period. Furthermore, a substantial decrease in vaccinees’ anti-Spike antibodies binding to Spike protein of Omicron VOC was also observed. Critically ill COVID-19 patients had higher levels of anti-Spike and anti-Nucleocapsid antibodies compared to HCWs. No significant differences in anti-Spike and anti-Nucleocapsid antibody levels between the critically ill COVID-19 patients that were on non-invasive oxygen supplementation and those on invasive ventilation support were observed. However, stronger anti-Spike, but not anti-Nucleocapsid, antibody response correlated with a better disease outcome in the cohort of patients on invasive ventilation support. Altogether, our results contribute to the growing pool of data on humoral responses to SARS-CoV-2 infection and vaccination.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleViruses
Numberingvol. 14, no. 9, 1966
e-ISSN1999-4915
DOIhttps://doi.org/10.3390/v14091966
URN:NBNurn:nbn:hr:184:927357
Publication2022-09-04
ProjectNumber: IP-CORONA-2020-04-2073
Title (croatian): Dosta skrivanja CoV-2: uspostava baze monoklonskih protutijela na SARS-Cov-2
Title (english): No more hiding CoV-2: Establishment of SARS-Cov-2 mAbeom
Acronym: CoV2mAbs
Leader: Ilija Brizić
Jurisdiction: Croatia
Funding stream: IP-CORONA
Document URLhttps://www.mdpi.com/1999-4915/14/9/1966
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-10-10 10:56:06