prikaz prve stranice dokumenta Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
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Scientific paper - Original scientific paper
Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
International Journal of Molecular Sciences, 24 (2023), 5; 4751. https://doi.org/10.3390/ijms24054751
Valenčić Seršić, Lara; Krušić Alić, Vedrana; Biberić, Maša; Zrna, Siniša; Jagoić, Tin; Tarčuković, Janja; Grabušić, Kristina

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Identifikacija cirkulirajućih biomarkera neurološkog oporavka u bolesnika s ozljedom mozga: zbirka radova | Virtual collection

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Valenčić Seršić, L., Krušić Alić, V., Biberić, M., Zrna, S., Jagoić, T., Tarčuković, J. & Grabušić, K. (2023). Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury. International Journal of Molecular Sciences, 24. (5). doi: 10.3390/ijms24054751

Valenčić Seršić, Lara, et al. "Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury." International Journal of Molecular Sciences, vol. 24, no. 5, 2023. https://doi.org/10.3390/ijms24054751

Valenčić Seršić, Lara, Vedrana Krušić Alić, Maša Biberić, Siniša Zrna, Tin Jagoić, Janja Tarčuković and Kristina Grabušić. "Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury." International Journal of Molecular Sciences 24, no. 5 (2023). https://doi.org/10.3390/ijms24054751

Valenčić Seršić, L., et al. (2023) 'Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury', International Journal of Molecular Sciences, 24(5). doi: 10.3390/ijms24054751

Valenčić Seršić L, Krušić Alić V, Biberić M, Zrna S, Jagoić T, Tarčuković J, and sur.. Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury. International Journal of Molecular Sciences [Internet]. 2023 March 01 [cited 2024 May 22];24(5). doi: 10.3390/ijms24054751

L. Valenčić Seršić, et al., "Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury", International Journal of Molecular Sciences, vol. 24, no. 5, March 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:881493. [Accessed: 22 May 2024]

Metadata
Title (english)Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
AuthorLara Valenčić Seršić
AuthorVedrana Krušić Alić
AuthorMaša Biberić
AuthorSiniša Zrna
AuthorTin Jagoić
AuthorJanja Tarčuković
AuthorKristina Grabušić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anaesthesiology,reanimatology and Intensive Care)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Anesthesiology and Reanimatology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Intensive Care
Abstract (english)
Severe traumatic brain injury (sTBI) is an intracranial damage triggered by external force, most commonly due to falls and traffic accidents. The initial brain injury can progress into a secondary injury involving numerous pathophysiological processes. The resulting sTBI dynamics makes the treatment challenging and prompts the improved understanding of underlying intracranial processes. Here, we analysed how extracellular microRNAs (miRNAs) are affected by sTBI. We collected thirty-five cerebrospinal fluids (CSF) from five sTBI patients during twelve days (d) after the injury and combined them into d1–2, d3–4, d5–6 and d7–12 CSF pools. After miRNA isolation and cDNA synthesis with added quantification spike-ins, we applied a real-time PCR-array targeting 87 miRNAs. We detected all of the targeted miRNAs, with totals ranging from several nanograms to less than a femtogram, with the highest levels found at d1–2 followed by decreasing levels in later CSF pools. The most abundant miRNAs were miR-451a, miR-16-5p, miR-144-3p, miR-20a-5p, let-7b-5p, miR-15a-5p, and miR-21-5p. After separating CSF by size-exclusion chromatography, most miRNAs were associated with free proteins, while miR-142-3p, miR-204-5p, and miR-223-3p were identified as the cargo of CD81-enriched extracellular vesicles, as characterised by immunodetection and tunable resistive pulse sensing. Our results indicate that miRNAs might be informative about both brain tissue damage and recovery after sTBI.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 24, no. 5, 4751
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms24054751
URN:NBNurn:nbn:hr:184:881493
Publication2023-03-01
ProjectNumber: IP-2019-04-1511
Title (croatian): Identifikacija cirkulirajućih biomarkera neurološkog oporavka u bolesnika s ozljedom mozga
Title (english): Identification of circulating biomarkers for neuro-recovery of brain-injured patients
Acronym: CiMozak / CirBrain
Leader: Kristina Grabušić
Jurisdiction: Croatia
Funding stream: IP
ProjectNumber: uniri-biomed-18-5
Title (croatian): Izvanstanične vezikule kao klinički markeri neuroregeneracije nakon teške ozljede mozga
Title (english): Extracellular vesicles as clinical markers for neuroregeneration after severe traumatic brain injury
Leader: Kristina Grabušić
Jurisdiction: Croatia
Funding stream: Sveučilište u Rijeci
Document URLhttps://www.mdpi.com/1422-0067/24/5/4751
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-03-03 07:00:35