prikaz prve stranice dokumenta MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment
Access restricted to all Croatian higher education institution's students and staff
Conference presentation
MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment
At: 2021 Annual Meeting of the Croatian Immunological Society. Trogir, Croatia, 2021. urn:nbn:hr:184:924123
Pavišić, Valentino; Mahmutefendić Lučin, Hana; Gulić, Tamara; Jug Vučko, Natalia; Lučin, Pero; Blagojević Zagorac, Gordana

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Pavišić, V., Mahmutefendić Lučin, H., Gulić, T., Jug Vučko, N., Lučin, P. & Blagojević Zagorac, G. MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment [PowerPoint slides]. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:924123

Pavišić, Valentino, et al. "MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment." 2021 Annual Meeting of the Croatian Immunological Society, 23.09.2021.-25.09.2021., Trogir, Croatia. Presentation. https://urn.nsk.hr/urn:nbn:hr:184:924123

Pavišić, Valentino, Hana Mahmutefendić Lučin, Tamara Gulić, Natalia Jug Vučko, Pero Lučin and Gordana Blagojević Zagorac. "MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment." Presentation at 2021 Annual Meeting of the Croatian Immunological Society, Trogir, Croatia, 23.09.2021.-25.09.2021. https://urn.nsk.hr/urn:nbn:hr:184:924123

Pavišić, V., et al. 'MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment' [Presentation], 2021 Annual Meeting of the Croatian Immunological Society. 23.09.2021.-25.09.2021. Available at: https://urn.nsk.hr/urn:nbn:hr:184:924123

Pavišić V, Mahmutefendić Lučin H, Gulić T, Jug Vučko N, Lučin P, Blagojević Zagorac G. MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment. [Presentation] [cited 2024 May 02] Available at: https://urn.nsk.hr/urn:nbn:hr:184:924123

V. Pavišić, H. Mahmutefendić Lučin, T. Gulić, N. Jug Vučko, P. Lučin and G. Blagojević Zagorac, "MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment", presented at: 2021 Annual Meeting of the Croatian Immunological Society, Trogir, 23.09.2021.-25.09.2021. Available at: https://urn.nsk.hr/urn:nbn:hr:184:924123

Metadata
Title (english)MCMV induces activation and accumulation of Arf6 GTPase on membranes of virion assembly compartment
AuthorValentino Pavišić
AuthorHana Mahmutefendić Lučin
AuthorTamara Gulić
AuthorNatalia Jug Vučko
AuthorPero Lučin
AuthorGordana Blagojević Zagorac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Shortly after entering the host cells, murine cytomegaloviruses (MCMVs) reorganize Golgi and endosomal system of the infected cells and form the MCMV assembly compartment (AC) in order to enable production of the new virions. One of the features of the reorganized membrane organelles in AC is the extensive tubulation that in uninfected cells, among other GTPases from Ras family, is triggered by Arf GTPases, especially Arf6. The aim of this study was to determine expression, localization, as well as degree of activation of Arf6 and its main regulators (GAPs and GEFs) during MCMV infection. In order to address this question, Balb 3T3 cells were infected with recombinant murine cytomegalovirus Δm138-MCMV and expression of Arf6, its regulators, and MCMV viral proteins was followed up to 30 hours post infection (30 hpi) by Western blot and by confocal microscopy. Degree of Arf6 activation was determined by pull-down and wound healing assays. Viral replication was monitored in cells infected with C3X-GFP MCMV. Up to 30 hpi, there are no significant changes in Arf6 expression, although its intracellular localization and activation are altered by MCMV infection, as are the levels and intracellular localization of its major regulators.
Languageenglish
Peer reviewUnknown
URN:NBNurn:nbn:hr:184:924123
Presentation date2021-09-23
ProjectNumber: HRZZ-2020-2-1323
Title (croatian): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Leader: Gordana Blagojević Zagorac
Jurisdiction: Croatia
ConferenceTitle: 2021 Annual Meeting of the Croatian Immunological Society
Acronym: HID
Location: Trogir, Croatia
Organizer: HID
Start date: 2021-09-23
End date: 2021-09-25
Assembly type: Lecture
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2023-04-05 08:00:59