prikaz prve stranice dokumenta BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION
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BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION
At: Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge. Brela, Croatia, 2022. urn:nbn:hr:184:999161
Blagojević Zagorac, Gordana; Karleuša, Ljerka; Viduka, Ivona; Jug Vučko, Natalia; Pavišić, Valentino; Mahmutefendić Lučin, Hana; Lučin, Pero

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Blagojević Zagorac, G., Karleuša, Lj., Viduka, I., Jug Vučko, N., Pavišić, V., Mahmutefendić Lučin, H. & Lučin, P. BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION [PowerPoint slides]. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac, Gordana, et al. "BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION." Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge, 28.09.2022.-01.10.2022., Brela, Croatia. Presentation. https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac, Gordana, Ljerka Karleuša, Ivona Viduka, Natalia Jug Vučko, Valentino Pavišić, Hana Mahmutefendić Lučin and Pero Lučin. "BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION." Presentation at Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge, Brela, Croatia, 28.09.2022.-01.10.2022. https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac, G., et al. 'BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION' [Presentation], Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge. 28.09.2022.-01.10.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac G, Karleuša Lj, Viduka I, Jug Vučko N, Pavišić V, Mahmutefendić Lučin H, and sur.. BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION. [Presentation] [cited 2024 May 02] Available at: https://urn.nsk.hr/urn:nbn:hr:184:999161

G. Blagojević Zagorac, et al., "BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION", presented at: Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge, Brela, 28.09.2022.-01.10.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:999161

Metadata
Title (english)BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION
AuthorGordana Blagojević Zagorac
AuthorLjerka Karleuša
AuthorIvona Viduka
AuthorNatalia Jug Vučko
AuthorValentino Pavišić
AuthorHana Mahmutefendić Lučin
AuthorPero Lučin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Arf proteins are small GTPases that cycle between active (GTP) and inactive (GDP) forms. Their activation is mediated by guanine nucleotide exchange factors (GEFs), and their inactivation is facilitated by GTPase-activating proteins (GAPs). Since we have previously shown that Arf1 and Arf6 proteins are required for the establishment of murine cytomegalovirus (MCMV) infection, the aim of this study was to determine importance of Arf GEFs in regulation of Arf´s activity during the immediate-early and early phase of MCMV infection. Balb 3T3 murine fibroblasts treated with small inhibitors of Arf GEFs were infected with MCMV and the expression of viral proteins was determined by Western blot and immunofluorescence microscopy after 6 and 16 hours of infection. In addition, cells were infected with C3X-GFP MCMV, in which a GFP expression cassette was inserted upstream of the ie2 gene, and the intensity of green fluorescence as well as percentage of GFP positive cells was monitored by flow cytometry as an indicators for progression of the immediate-early phase of infection. The expression of IE1, E1 and m06 proteins was reduced in cells treated with Brefeldin A (BFA), Golgicid A (GCA), NAV2729 or EXO2, in contrast to cells treated with SecinH3 or EXO1 in which the expression of the viral proteins was similar to that in control cells. In cells infected with C3X-GFP MCMV, percentage of GFP positive cells and the green fluorescence intensity were significantly decreased in cells treated with BFA, GCA and EXO2 compared with control cells, suggesting that inhibition of Gbf1, Big1, Big2 and BRAG2 Arf GEFs prevents entry into the immediate–early/early phase of MCMV gene expression. Our results suggest that Arf GEFs from Big, Gbf and Brag2 family have important role in regulation of Arf protein activity during the earliest stages of MCMV infection and are required for the progression of immediate-early and early phase of MCMV infection.
Languageenglish
Peer reviewUnknown
URN:NBNurn:nbn:hr:184:999161
Presentation date2022-10-01
ProjectNumber: IP-2020-02-1323
Title (croatian): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Leader: Gordana Blagojević zagorac
Jurisdiction: Croatia
ProjectNumber: IP-2019-04-3582
Title (croatian): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Leader: Pero Lučin
Jurisdiction: Croatia
ConferenceTitle: Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge
Location: Brela, Croatia
Start date: 2022-09-28
End date: 2022-10-01
Assembly type: Lecture
Type of resourceText
Access conditionsRestricted access
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Created on2023-04-05 11:52:52