prikaz prve stranice dokumenta Activation of Arf GTPases during murine cytomegalovirus infection
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Conference presentation
Activation of Arf GTPases during murine cytomegalovirus infection
At: Annual meeting of the Croatian Immunological Society 2022. Sveti Martin na Muri, Croatia, 2022. urn:nbn:hr:184:023279
Blagojević Zagorac, Gordana; Gulić, Tamara; Pavišić, Valentino; Mahmutefendić Lučin, Hana; Lukanović Jurić, Silvija; Lučin, Pero

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Blagojević Zagorac, G., Gulić, T., Pavišić, V., Mahmutefendić Lučin, H., Lukanović Jurić, S. & Lučin, P. Activation of Arf GTPases during murine cytomegalovirus infection [PowerPoint slides]. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:023279

Blagojević Zagorac, Gordana, et al. "Activation of Arf GTPases during murine cytomegalovirus infection." Annual meeting of the Croatian Immunological Society 2022, 23.09.2022.-25.09.2022., Sveti Martin na Muri, Croatia. Presentation. https://urn.nsk.hr/urn:nbn:hr:184:023279

Blagojević Zagorac, Gordana, Tamara Gulić, Valentino Pavišić, Hana Mahmutefendić Lučin, Silvija Lukanović Jurić and Pero Lučin. "Activation of Arf GTPases during murine cytomegalovirus infection." Presentation at Annual meeting of the Croatian Immunological Society 2022, Sveti Martin na Muri, Croatia, 23.09.2022.-25.09.2022. https://urn.nsk.hr/urn:nbn:hr:184:023279

Blagojević Zagorac, G., et al. 'Activation of Arf GTPases during murine cytomegalovirus infection' [Presentation], Annual meeting of the Croatian Immunological Society 2022. 23.09.2022.-25.09.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:023279

Blagojević Zagorac G, Gulić T, Pavišić V, Mahmutefendić Lučin H, Lukanović Jurić S, Lučin P. Activation of Arf GTPases during murine cytomegalovirus infection. [Presentation] [cited 2024 May 02] Available at: https://urn.nsk.hr/urn:nbn:hr:184:023279

G. Blagojević Zagorac, T. Gulić, V. Pavišić, H. Mahmutefendić Lučin, S. Lukanović Jurić and P. Lučin, "Activation of Arf GTPases during murine cytomegalovirus infection", presented at: Annual meeting of the Croatian Immunological Society 2022, Sveti Martin na Muri, 23.09.2022.-25.09.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:023279

Metadata
Title (english)Activation of Arf GTPases during murine cytomegalovirus infection
AuthorGordana Blagojević Zagorac
AuthorTamara Gulić
AuthorValentino Pavišić
AuthorHana Mahmutefendić Lučin
AuthorSilvija Lukanović Jurić
AuthorPero Lučin
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Arf proteins are small GTP-binding proteins (GTPases) that are one of the main regulators of endocytosis and intracellular trafficking. They function as molecular switches, meaning that they continuously cycle between active (GTP) and inactive (GDP) form. When activated they bind to the membranes of endosomal compartments where they recruit their effector molecules. We have previously shown that Arf GTPases have important role in pathogenesis of murine cytomegalovirus (MCMV) infection. While Arf1 and Arf6 are important for the establishment of MCMV infection, Arf3 and Arf4 have important role in biogenesis of virion assembly compartment. The aim of this study was to determine the degree of Arf GTPases activation during MCMV infection. For that purpose, Balb 3T3 cells were infected with ∆m138 MCMV and at 0, 6, 16 and 30 hours post infection degree of Arf GTPases activation was determined by 3 different methods: subcellular fractionation, precipitation of Arf proteins with GGA3 PBD agarose beads and by analysing intracellular localization of Arf effector molecules. Our results have shown that the main effector molecules of Arf proteins are accumulated in the juxtanuclear area of MCMV infected cells with a pattern like that of the Arf proteins. Furthermore, the proportion of Arf proteins that are precipitated with GGA3 PBD agarose beads increases with progression of MCMV infection, suggesting that Arf proteins are overactivated during MCMV infection, although we did not observe an increase in membrane fraction of Arf proteins.
Languageenglish
Peer reviewUnknown
URN:NBNurn:nbn:hr:184:023279
Presentation date2022-09-24
ProjectNumber: IP-2020-02-1323
Title (croatian): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Leader: Gordana Blagojević Zagorac
Jurisdiction: Croatia
ProjectNumber: IP-2019-04-3582
Title (croatian): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Leader: Pero Lučin
Jurisdiction: Croatia
ConferenceTitle: Annual meeting of the Croatian Immunological Society 2022
Location: Sveti Martin na Muri, Croatia
Start date: 2022-09-23
End date: 2022-09-25
Assembly type: Lecture
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2023-04-05 13:06:00