prikaz prve stranice dokumenta Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer
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Scientific paper - Original scientific paper
Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer
Scandinavian Journal of Immunology, 74 (2011), 4; 368-376. https://doi.org/10.1111/j.1365-3083.2011.02569.x
Sotošek Tokmadžić, Vlatka; Tomaš, M.I.; Sotošek, Stanislav; Laškarin, Gordana; Tulić, V.; Đorđević, Gordana; Šustić, Alan; Mrakovčić-Šutić, Ines

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Sotošek Tokmadžić, V., Tomaš, M., Sotošek, S., Laškarin, G., Tulić, V., Đorđević, G. ... Mrakovčić Šutić, I. (2011). Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer. Scandinavian Journal of Immunology, 74. (4), 368-376. doi: 10.1111/j.1365-3083.2011.02569.x

Sotošek Tokmadžić, Vlatka, et al. "Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer." Scandinavian Journal of Immunology, vol. 74, no. 4, 2011, pp. 368-376. https://doi.org/10.1111/j.1365-3083.2011.02569.x

Sotošek Tokmadžić, Vlatka, M.I. Tomaš, Stanislav Sotošek, Gordana Laškarin, V. Tulić, Gordana Đorđević, Alan Šustić and Ines Mrakovčić Šutić. "Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer." Scandinavian Journal of Immunology 74, no. 4 (2011): 368-376. https://doi.org/10.1111/j.1365-3083.2011.02569.x

Sotošek Tokmadžić, V., et al. (2011) 'Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer', Scandinavian Journal of Immunology, 74(4), pp. 368-376. doi: 10.1111/j.1365-3083.2011.02569.x

Sotošek Tokmadžić V, Tomaš M, Sotošek S, Laškarin G, Tulić V, Đorđević G, and sur.. Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer. Scandinavian Journal of Immunology [Internet]. 2011 October [cited 2024 April 29];74(4):368-376. doi: 10.1111/j.1365-3083.2011.02569.x

V. Sotošek Tokmadžić, et al., "Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer", Scandinavian Journal of Immunology, vol. 74, no. 4, pp. 368-376, October 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:227210. [Accessed: 29 April 2024]

Metadata
Title (english)Different Perforin Expression in Peripheral Blood and Prostate Tissue in Patients with Benign Prostatic Hyperplasia and Prostate Cancer
AuthorVlatka Sotošek Tokmadžić
AuthorM.I. Tomaš
AuthorStanislav Sotošek
AuthorGordana Laškarin
AuthorV. Tulić
AuthorGordana Đorđević
AuthorAlan Šustić
AuthorInes Mrakovčić-Šutić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anaesthesiology,reanimatology and Intensive Care)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of General Pathology and Pathological Anatomy)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Urology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Perforin (P) is a prototypical cytotoxic molecule involved in cell-mediatedimmunity against various pathogens, alloantigens and particularly differenttumours. The purpose of this study was to determine P expression in differentlymphocyte subpopulations isolated from peripheral blood and prostate tissueof patients with benign prostatic hyperplasia (BPH) and prostate cancer (PCa)and compare it with the P expression found in the control group. Twenty sub-jects were recruited in each of the groups. Prostate mononuclear cells of theBPH and PCa tissues were isolated by enzymatic digestion and gradient den-sity centrifugation, whereas peripheral blood mononuclear cells were isolatedby gradient density centrifugation alone. Cells and tissue samples were labelledusing monoclonal antibodies against P and different surface antigens (CD3,CD4, CD8 and CD56) and analysed by immunofluorescence and flow cytome-try. Total P expression in peripheral blood lymphocytes did not differ signifi-cantly between BPH⁄PCa patients and control group, although the BPH andPCa tissue showed lower P expression level. A negative correlation betweenprostate-specific antigen levels and the overall percentage of P+, CD3+CD56)P+, and CD3)CD56+P+cells in the prostate tissue was observed onlyin patients with PCa. Our findings indicate that the low frequency of P+lym-phocytes, including T, NKT and NK cells, in the prostate tissue of patientswith BPH and, particularly, PCa could be the consequence of local tissuemicroenvironment and one of the mechanisms involved in the pathogenesis ofprostate hyperplasia following malignant alteration.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleScandinavian Journal of Immunology
Numberingvol. 74, no. 4, pp. 368-376
p-ISSN0300-9475
DOIhttps://doi.org/10.1111/j.1365-3083.2011.02569.x
URN:NBNurn:nbn:hr:184:227210
Publication2011-10
Document URLhttps://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2011.02569.x
Type of resourceText
Access conditionsInstitutional access
Terms of useLicence In copyright icon
Created on2023-04-28 13:10:25