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Professional paper - Review paper
Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze
Medicina Fluminensis : Medicina Fluminensis, 51 (2015), 2; 296-304. urn:nbn:hr:184:146757
Tićac, Brigita; Mahmić, Jasmina; Barac, Sandra; Rožmanić, Vojko

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Tićac, B., Mahmić, J., Barac, S. & Rožmanić, V. (2015). Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze. Medicina Fluminensis : Medicina Fluminensis, 51. (2), 296-304. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:146757

Tićac, Brigita, et al. "Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze." Medicina Fluminensis : Medicina Fluminensis, vol. 51, no. 2, 2015, pp. 296-304. https://urn.nsk.hr/urn:nbn:hr:184:146757

Tićac, Brigita, Jasmina Mahmić, Sandra Barac and Vojko Rožmanić. "Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze." Medicina Fluminensis : Medicina Fluminensis 51, no. 2 (2015): 296-304. https://urn.nsk.hr/urn:nbn:hr:184:146757

Tićac, B., et al. (2015) 'Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze', Medicina Fluminensis : Medicina Fluminensis, 51(2), pp. 296-304. Available at: https://urn.nsk.hr/urn:nbn:hr:184:146757 (Accessed 14 May 2024)

Tićac B, Mahmić J, Barac S, Rožmanić V. Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze. Medicina Fluminensis : Medicina Fluminensis [Internet]. 2015 June 01 [cited 2024 May 14];51(2):296-304. Available at: https://urn.nsk.hr/urn:nbn:hr:184:146757

B. Tićac, J. Mahmić, S. Barac and V. Rožmanić, "Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze", Medicina Fluminensis : Medicina Fluminensis, vol. 51, no. 2, pp. 296-304, June 2015. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:146757. [Accessed: 14 May 2024]

Metadata
Title (croatian)Primjena testa otpuštanja interferona gama (IGRA) u imunodijagnostici tuberkuloze
Title (english)The use of an interferon gamma release assay (IGRA) in the immunodiagnosis of tuberculosis
AuthorBrigita Tićac
AuthorJasmina Mahmić
AuthorSandra Barac
AuthorVojko Rožmanić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Pediatrics
Abstract (croatian)
Cilj: Otkrivanje latentne tuberkuloze sukladno nacionalnim smjernicama uključuje procjenu rizika, radiografsku pretragu, izvođenje tuberkulinskog kožnog / PPD testa (PPD – purificirani proteinski derivat) i/ili novijeg ex vivo testa otpuštanja interferona gama (engl. Interferon Gamma Release Assay – IGRA). U radu je analizirana dinamika izvođenja i rezultati dobiveni dijagnostičkom primjenom imunoenzimske IGRA pretrage na M. tuberculosis. Metode: Izvršena je retrospektivna analiza rezultata Quantiferon-TB Gold In-Tube (QFT-GIT) testiranja provedenih u Laboratoriju za dijagnostiku tuberkuloze Nastavnog zavoda za javno zdravstvo Primorsko-goranske županije (Hrvatska). Rezultati: U sedmogodišnjem razdoblju (2007. – 2013.) u Laboratoriju za dijagnostiku tuberkuloze primjenom QFT-GIT testa analizirano je 2.110 uzoraka krvi pacijenata koji su pripadali različitim dobnim skupinama (od dobi od mjesec dana do 95 godina; x̄ = 41,9 godina; SD = 29,1; mod 56 godina). Rezultati analize su bili pozitivni u 585 (27,7 %), negativni u 1.498 (71 %) uzoraka, a u 27 (1,3 %) izvršenih pretraga su bili neodredivi. Pozitivni nalazi podjednako su zastupljeni u pretragama izvršenim za muškarce (29 % od 1.058 pretraga) i žene (26 % od 1.052 pretrage). Veća zastupljenost pozitivnih nalaza zabilježena je u osoba starijih od 56 godina, napose u dobnoj skupini od 81 do 85 godina (61 % od 44 pretrage). Prema uputnoj dijagnozi pozitivni nalazi su zastupljeniji kod hiperreaktora (40,5 % od 84 analize), TB kontakata (35,1 % od 464 analize) i zdravstvenih radnika (34,9 % od 43 analize). Rasprava i zaključci: Primjena in vitro IGRA QFT-GIT testa u kombinaciji s konvencionalnim metodama danas je prepoznata kao dijagnostički postupak koji ima značajnu ulogu u otkrivanju LTB-a, što predstavlja važan korak u prevenciji tuberkuloze, posebice kod rizičnih skupina.
Abstract (english)
Aim: Tuberculosis (TB) remains a major global public health problem. Screening for latent tuberculosis infection (LTB) usually involves risk assessment, chest X-ray, the tuberculin skin/PPD (Purified Protein Derivate) test, and/or interferon-gamma release assay (IGRA), according to country-specific guidelines. This paper describes the frequency of application and the results obtained by enzyme-linked immunosorbent IGRA assay for M. tuberculosis. Methods: We conducted a retrospective analysis of the results obtained using Quantiferon-TB Gold In-Tube (QFT-GIT) analysis at the Laboratory for the diagnosis of tuberculosis – Teaching Institute of Public Health County Primorsko-goranska (Croatia). Results: In the seven-year period (2007–2013) QFT-GIT analysis were processed in 2110 blood samples of patients from different age groups (aged 1 month-95 years; x̄ = 41.9 years; SD = 29.1; mode 56 years). Results were positive in 585 (27.7 %) samples, whereas negative results were found in 1498 (71 %) cases, and in 27 (1.3 %) samples the results were indeterminate. Positive results were distributed equally among males (29 % of 1058 samples) and females (26 % of 1052 samples). Higher incidence of positive results was observed in people over the age of 56, especially in the age group between 81-85 years (61 % of 44 tests). Positive results were most common in the group of hyperreactors (40.5 % of 84 tests) then in TB contacts (35.1 % of 464 tests), as well as health workers (34.9 % of 43 tests). Discussion and Conclusions: Applying in vitro IGRA QFT-GIT test in combination with conventional methods is recognized as a diagnostic method that plays an important role in uncovering LTB and as such represents an important step in prevention of tuberculosis, especially in high-risk groups.
Keywords (croatian)
Keywords (english)
Languagecroatian
Publication typeProfessional paper - Review paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleMedicina Fluminensis : Medicina Fluminensis
Numberingvol. 51, no. 2, pp. 296-304
p-ISSN0025-7729
URN:NBNurn:nbn:hr:184:146757
Publication2015-06-01
Document URLhttp://hrcak.srce.hr/139476
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-25 11:00:27