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Scientific paper - Original scientific paper
Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva
Medicina Fluminensis : Medicina Fluminensis, 44 (2008), 1; 60-66. urn:nbn:hr:184:172383
Marić, Ivana; Kučić, Natalia; Grahovac, Blaženka; Bobinac, Dragica; Vukičević, Slobodan

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Marić, I., Kučić, N., Grahovac, B., Bobinac, D. & Vukičević, S. (2008). Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva. Medicina Fluminensis : Medicina Fluminensis, 44. (1), 60-66. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:172383

Marić, Ivana, et al. "Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva." Medicina Fluminensis : Medicina Fluminensis, vol. 44, no. 1, 2008, pp. 60-66. https://urn.nsk.hr/urn:nbn:hr:184:172383

Marić, Ivana, Natalia Kučić, Blaženka Grahovac, Dragica Bobinac and Slobodan Vukičević. "Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva." Medicina Fluminensis : Medicina Fluminensis 44, no. 1 (2008): 60-66. https://urn.nsk.hr/urn:nbn:hr:184:172383

Marić, I., et al. (2008) 'Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva', Medicina Fluminensis : Medicina Fluminensis, 44(1), pp. 60-66. Available at: https://urn.nsk.hr/urn:nbn:hr:184:172383 (Accessed 10 November 2024)

Marić I, Kučić N, Grahovac B, Bobinac D, Vukičević S. Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva. Medicina Fluminensis : Medicina Fluminensis [Internet]. 2008 June 01 [cited 2024 November 10];44(1):60-66. Available at: https://urn.nsk.hr/urn:nbn:hr:184:172383

I. Marić, N. Kučić, B. Grahovac, D. Bobinac and S. Vukičević, "Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva", Medicina Fluminensis : Medicina Fluminensis, vol. 44, no. 1, pp. 60-66, June 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:172383. [Accessed: 10 November 2024]

Metadata
Title (croatian)Izražaj koštanog morfogenetskog proteina -2 i -7 tijekom eksperimentalne upalne bolesti crijeva
Title (english)Expression of bone morphogenetic protein -2 and -7 during experimental inflammatory bowel disease
AuthorIvana Marić
AuthorNatalia Kučić
AuthorBlaženka Grahovac
AuthorDragica Bobinac
AuthorSlobodan Vukičević
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anatomy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Anatomy
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract (croatian)
SAŽETAK. Cilj: Za koštane morfogenetske proteine -2 i -7 pokazano je da osim snažnog osteoinduktivnog svojstva imaju važnu ulogu u razvoju i cijeljenju različitih neskeletnih tkiva i organa. Svrha ovog rada je ispitati ekspresiju BMP-2 i BMP-7 tijekom upalne bolesti crijeva u štakora, te učinak dvotjednog liječenja sa BMP-7 na cijeljenje tkiva kolona i ekspresiju ovih morfogenetskih proteina. Metode: Eksperimentalni kolitis izazvan je primjenom klizme koja sadrži 2,4,6-trinitrobenzensulfoničnu kiselinu (TNBS) i etanol. Na uzorcima crijeva pratili smo makroskopske i mikroskopske promjene koje se zbivaju tijekom akutne i kronične faze kolitisa, te nakon i.v. primjene BMP-7. Metodom imunohistokemije i lančane reakcije polimeraze pratili smo izražaj BMP-2 i -7. Rezultati: Imunohistokemijska i RT-PCR analiza pokazala je ekspresiju BMP-2 i -7 tijekom akutne i kronične faze upalne bolesti crijeva. U akutnoj fazi kolitisa BMP-2 i -7 prisutni su u području ulkusa, a u kroničnoj fazi BMP-7 pozitivno bojanje prisutno je u kolonocitima uzduž kripti kolona, dok je BMP-2 pozitivno bojanje u kolonocitima površnog epitela te između kripta. Specifično bojanje na BMP-7 kao i ekspresija mRNA za BMP-7 na uzorcima crijeva životinja liječenim sa BMP-7 pokazuje smanjenje prisustva BMP-7, a pojačano bojanje i ekspresiju mRNA za BMP-2. Zaključak: Sistemska primjena koštanog morfogenetskog proteina-7 smanjuje makroskopsko i mikroskopsko oštećenje kolona tijekom upalne bolesti crijeva u štakora. Način ekspresije BMP-2 i -7 tijekom upalne bolesti crijeva te nakon liječenja sa BMP-7 ukazuje na značajnu ulogu koštanih morfogenetskih proteina u kontroli oštećenja i cijeljenja tkiva kolona tijekom ove bolesti.
Abstract (english)
ABSTRACT. Aim: Bone morphogenetic proteins -2 and -7, along with their osteoinductive function, play important role in the development and healing of different non-skeletal tissues and organs. The aim of this study was to reveal the expression of BMP-2 and BMP-7 during experimental inflammatory bowel disease, as well as after 14th day of BMP-7 treatment. Methods: Experimental colitis was induced by the intracolonic administration of the clysma containing 2,4,6-trinitrobenzensulphonic acid (TNBS) and ethanol. We investigated the macroscopic and microscopic colon tissue damage during acute and chronic phases of colitis as well as after IV treatment of BMP-7. Results: Imunohistochemical and RT-PCR analyses showed expression of BMP-2 and -7 during acute and chronic phases of inflammatory bowel disease. In the acute phase of colitis, BMP-2 and -7 expressions were located in mucosa of ulcer region. In the chronic phase BMP-7 expression was placed in the colonocytes along the crypt, while BMP-2 expression was located in the coloncytes of surface epithelium and intercrypt table. BMP-7 treatment reduced the expression of BMP-7 and mRNA for BMP-7 but also increased the expression of BMP-2 and mRNA for BMP-2 in colonic tissue. Conclusions: Systemic administration of bone morphogenetic protein 7 decreased macroscopic and microscopic colonic tissue damage during experimental inflammatory bowel disease. The expression pattern of BMP-2 and -7 during inflammatory bowel disease, as well as after BMP-7 treatment suggests an important role of bone morphogenetic proteins in control of colon tissue damage and healing during disease.
Keywords (croatian)
Keywords (english)
Languagecroatian
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleMedicina Fluminensis : Medicina Fluminensis
Numberingvol. 44, no. 1, pp. 60-66
p-ISSN0025-7729
URN:NBNurn:nbn:hr:184:172383
Publication2008-06-01
Document URLhttp://hrcak.srce.hr/25952
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-28 09:02:46