prikaz prve stranice dokumenta Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections
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Scientific paper - Original scientific paper
Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections
Antibiotics, 13 (2024), 9; 829. https://doi.org/10.3390/antibiotics13090829
Repac Antić, Davorka; Kovač, Bruno; Kolenc, Marko; Brčić Karačonji, Irena; Gobin, Ivana; Petković Didović, Mirna

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Repac Antić, D., Kovač, B., Kolenc, M., Brčić Karačonji, I., Gobin, I. & Petković Didović, M. (2024). Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections. Antibiotics, 13. (9). doi: 10.3390/antibiotics13090829

Repac Antić, Davorka, et al. "Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections." Antibiotics, vol. 13, no. 9, 2024. https://doi.org/10.3390/antibiotics13090829

Repac Antić, Davorka, Bruno Kovač, Marko Kolenc, Irena Brčić Karačonji, Ivana Gobin and Mirna Petković Didović. "Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections." Antibiotics 13, no. 9 (2024). https://doi.org/10.3390/antibiotics13090829

Repac Antić, D., et al. (2024) 'Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections', Antibiotics, 13(9). doi: 10.3390/antibiotics13090829

Repac Antić D, Kovač B, Kolenc M, Brčić Karačonji I, Gobin I, Petković Didović M. Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections. Antibiotics [Internet]. 2024 September 01 [cited 2024 November 26];13(9). doi: 10.3390/antibiotics13090829

D. Repac Antić, B. Kovač, M. Kolenc, I. Brčić Karačonji, I. Gobin and M. Petković Didović, "Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections", Antibiotics, vol. 13, no. 9, September 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:139298. [Accessed: 26 November 2024]

Metadata
Title (english)Combinatory Effect of Nitroxoline and Gentamicin in the Control of Uropathogenic Enterococci Infections
AuthorDavorka Repac Antić
AuthorBruno Kovač
AuthorMarko Kolenc
AuthorIrena Brčić Karačonji
AuthorIvana Gobin
AuthorMirna Petković Didović
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Enterococcus faecalis, responsible for a majority of human and nosocomial enterococcal infections, is intrinsically resistant to aminoglycoside antibiotics (such as gentamicin, GEN), which must be used in a combined therapy to be effective. Nitroxoline (NTX) is an old antibiotic, underused for decades, but rediscovered now in an era of growing antibiotic resistance. In this in vitro study, the types of interactions between NTX and GEN on 29 E. faecalis strains were analyzed with an aim to find synergistic antimicrobial and antiadhesion combinations. Transmission electron microscopy (TEM) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to analyze changes in cell morphology and bacterial proteome after monotreatments and combined treatments. The results showed the synergistic effect for six combinations on eight strains, including the ATCC29212, and an additive effect for most strains. Combinations causing a complete inhibition of adhesion were established. Cell membrane integrity was affected by NTX, while combined NTX/GEN treatment caused dramatic changes in cell morphology. Upregulation of the expression of many proteins was established, with some emerging only after combined treatment. The results strongly imply that NTX has the potential for use in combined therapy with GEN against enterococci and it could further provide a substantial contribution to an ongoing fight against antimicrobial resistance and nosocomial infections.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleAntibiotics
Numberingvol. 13, no. 9, 829
e-ISSN2079-6382
DOIhttps://doi.org/10.3390/antibiotics13090829
URN:NBNurn:nbn:hr:184:139298
Publication2024-09-01
ProjectNumber: KK.01.1.1.02.0007
Title (croatian): Istraživačko-edukacijski centar za zdravstvenu i medicinsku ekologiju i zaštitu od zračenja – Rekonstrukcija i dogradnja Instituta za medicinska istraživanja i medicinu rada u Zagrebu
Title (english): Research and Educational Centre of Environmental Health and Radiation Protection – Reconstruction and Expansion of the Institute for Medical Research and Occupational Health in Zagreb
Acronym: ReC-IMI
Leader: Ana Lucić Vrdoljak
Jurisdiction: eu
Funding stream: European Regional Development Fund
Document URLhttps://www.mdpi.com/2079-6382/13/9/829
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-09-05 10:10:37