prikaz prve stranice dokumenta Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis
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Scientific paper - Original scientific paper
Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis
Journal of Functional Biomaterials, 15 (2024), 9; 270. https://doi.org/10.3390/jfb15090270
Čandrlić, Marija; Jerbić Radetić, Ana Terezija; Omrčen, Hrvoje; Franović, Barbara; Batičić, Lara; Gulić, Tamara; Čaljkušić-Mance, Tea; Zoričić Cvek, Sanja; Malešić, Lucija; Perić Kačarević, Željka; Cvijanović Peloza, Olga

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Čandrlić, M., Jerbić Radetić, A. T., Omrčen, H., Franović, B., Batičić, L., Gulić, T. ... Cvijanović Peloza, O. (2024). Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis. Journal of Functional Biomaterials, 15. (9). doi: 10.3390/jfb15090270

Čandrlić, Marija, et al. "Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis." Journal of Functional Biomaterials, vol. 15, no. 9, 2024. https://doi.org/10.3390/jfb15090270

Čandrlić, Marija, Ana Terezija Jerbić Radetić, Hrvoje Omrčen, Barbara Franović, Lara Batičić, Tamara Gulić, Tea Čaljkušić Mance, et al. "Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis." Journal of Functional Biomaterials 15, no. 9 (2024). https://doi.org/10.3390/jfb15090270

Čandrlić, M., et al. (2024) 'Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis', Journal of Functional Biomaterials, 15(9). doi: 10.3390/jfb15090270

Čandrlić M, Jerbić Radetić AT, Omrčen H, Franović B, Batičić L, Gulić T, and sur.. Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis. Journal of Functional Biomaterials [Internet]. 2024 September 18 [cited 2024 December 27];15(9). doi: 10.3390/jfb15090270

M. Čandrlić, et al., "Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis", Journal of Functional Biomaterials, vol. 15, no. 9, September 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:225078. [Accessed: 27 December 2024]

Metadata
Title (english)Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis
AuthorMarija Čandrlić
AuthorAna Terezija Jerbić Radetić
AuthorHrvoje Omrčen
AuthorBarbara Franović
AuthorLara Batičić
AuthorTamara Gulić
AuthorTea Čaljkušić-Mance
AuthorSanja Zoričić Cvek
AuthorLucija Malešić
AuthorŽeljka Perić Kačarević
AuthorOlga Cvijanović Peloza
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anatomy)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The aim of this study was to evaluate the efficacy of autologous dentin (AD), bovine xenograft (BX) and magnesium-enriched bovine xenograft (BX + Mg) in the healing of critical cranial bone defects (CCBDs) in rats. Eighty male Wistar rats were divided into four groups: BX, BX + Mg, AD and the control group (no intervention). Eight mm CCBDs were created and treated with the respective biomaterials. Healing was assessed 7, 15, 21 and 30 days after surgery by micro-computed tomography (micro-CT), real-time polymerase chain reaction (RT-PCR) and immunohistochemical analysis. Micro-CT analysis showed that AD had the highest bone volume and the least amount of residual biomaterial at day 30, indicating robust bone formation and efficient resorption. BX + Mg showed significant bone volume but had more residual biomaterial compared to AD. RT-PCR showed that the expression of osteocalcin (OC), the receptor activator of nuclear factor κB (RANK) and sclerostin (SOST), was highest in the AD group at day 21 and vascular endothelial growth factor (VEGF) at day 15, indicating increased osteogenesis and angiogenesis in the AD group. Immunohistochemical staining confirmed intense BMP-2/4 and SMAD-1/5/8 expression in the AD group, indicating osteoinductive properties. The favorable gene expression profile and biocompatibility of AD and BX + Mg make them promising candidates for clinical applications in bone tissue engineering. Further research is required to fully exploit their potential in regenerative surgery.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Functional Biomaterials
Numberingvol. 15, no. 9, 270
e-ISSN2079-4983
DOIhttps://doi.org/10.3390/jfb15090270
URN:NBNurn:nbn:hr:184:225078
Publication2024-09-18
ProjectNumber: IP-2020-02-7875
Title (croatian): Usporedba reparacijskog odgovora koštanog tkiva upotrebom dentina, ksenogenog biomaterijala i autologne kosti
Title (english): Comparison of the repair of bone tissue response using dentin, xenogeneic biomaterial and autologous bone
Acronym: MgXBiomBone
Leader: Olga Cvijanović Peloza
Jurisdiction: Croatia
Funding stream: Research Projects
Document URLhttps://www.mdpi.com/2079-4983/15/9/270
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-09-19 06:43:21