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master's thesis
Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša
Zagreb: University of Zagreb, School of Medicine, 2024. urn:nbn:hr:105:326334
Potočki, Vinka
University of Zagreb
School of Medicine
Croatian Institiute for Brain Research

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Potočki, V. (2024). Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:326334

Potočki, Vinka. "Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša." Master's thesis, University of Zagreb, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:105:326334

Potočki, Vinka. "Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša." Master's thesis, University of Zagreb, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:105:326334

Potočki, V. (2024). 'Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša', Master's thesis, University of Zagreb, School of Medicine, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:326334

Potočki V. Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2024 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:105:326334

V. Potočki, "Utjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:105:326334

Metadata
TitleUtjecaj membranskih gangliozida na leptinski receptor i Na⁺,K⁺-ATPazu u talamusu miša
Title (english)The effect of membrane gangliosides on leptin receptor and Na⁺,K⁺-ATPase in mouse thalamus
AuthorVinka Potočki
MentorKristina Mlinac Jerković (mentor)
Committee memberSvjetlana Kalanj-Bognar (predsjednik povjerenstva)
Committee memberDragana Fabris (član povjerenstva)
Committee memberKristina Mlinac Jerković (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Croatian Institiute for Brain Research)
Zagreb
Defense date and country2024-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract
Gangliozidi, složeni membranski glikosfingolipidi, sudjeluju u međustaničnim i unutarstaničnim interakcijama te imaju ključnu ulogu u patogenezi raznih neuroloških i neurodegenerativnih bolesti. Membranski protein, leptinski receptor (LepR), modulira ponašanje u hranjenju tako da utječe na signalizaciju i integraciju u mnogim područjima središnjeg živčanog sustava. Studije pokazuju da gangliozid a-serije mijenjaju aktivnost leptina interakcijom s LepR-om. Promjena sadržaja gangliozida također može utjecati na regulaciju funkcije Na⁺,K⁺-ATPaze (NKA), dok leptinski putovi mogu inhibirati NKA. Naš je cilj bio rasvijetliti kako izmijenjeni sastav gangliozida u staničnoj membrani utječe na ekspresiju LepR-a, ekspresiju i aktivnost NKA te koncentraciju kolesterola u mišjem talamusu. Koristili smo St8sia1 null model miša, kojemu nedostaje GD3-sintaza i stoga ne sintetizira b- i c-seriju gangliozida, već umjesto toga sintetizira više a-serije gangliozida u usporedbi s divljim tipom (WT) miševa. Ispitane su obje izoforme, 125 kDa i 100 kDa LepR-a. Analizirali smo proteinsku ekspresiju metodom Western blot za LepR i NKA u uzorcima talamusa. Osim toga, mjerili smo enzimsku aktivnost NKA kod St8sia1 null u usporedbi s WT miševima te izmjerili koncentraciju kolesterola u talamusu istih životinja s obzirom da je poznato da gangliozidni sastav utječe na metabolizam kolesterola, a kolesterol utječe na enzimsku aktivnost NKA. Rezultati su pokazali veće količine NKA i manje količine obje izoforme LepR-a u uzorcima talamusa kod St8sia1 null miševa u usporedbi s WT miševima. Enzimska aktivnost NKA bila je manja u uzorcima talamusa St8sia1 null miševa u usporedbi s WT miševima, dok je koncentracija kolesterola bila veća. Stoga zaključujemo da izmijenjen sastav gangliozida utječe na obilježja NKA i LepR-a, praćeno manjom enzimskom aktivnošću NKA u mišjem talamusu, sugerirajući značajnu povezanost između leptinskog receptora i NKA u raznolikom glikolipidnom okruženju.
Abstract (english)
Gangliosides, complex membrane glycosphingolipids, engage in a variety of inter- and intracellular interactions and play a crucial role in the pathogenesis of various neurological and neurodegenerative diseases. Another membrane constituent, leptin receptor (LepR), modulates feeding behavior by influencing signaling and integration in many regions of the central nervous system. Studies show that a-series gangliosides alter leptin activity by interacting with LepR. The redistribution of gangliosides can also influence the regulation of the Na⁺,K⁺-ATPase (NKA) function, whereas leptin pathways can inhibit NKA. Our aim was to deepen the understanding of how altered ganglioside profile impacts the expression of LepR, the expression and activity of NKA, as well as cholesterol concentration in mouse thalamus. We used the St8sia1 null mouse model, which lacks GD3 synthase and consequently does not synthesize b-series gangliosides, but instead synthesizes more a-series gangliosides compared to wild-type (WT) mice. Both isoforms, 125 kDa and 100 kDa of LepR, were explored. We used protein expression analysis by Western blotting for LepR and NKA in thalamus samples. In addition, the enzyme activity of NKA was measured in null compared to WT mice, as well as cholesterol concentration, since according to literature ganglioside composition affects cholesterol metabolism, and cholesterol affects NKA activity. Results revealed higher amounts of NKA and lower amounts of both LepR isoforms in thalamus samples of the null mice compared to their WTs. Enzyme activity of NKA was lower in thalamus samples of null mice compared to their WTs, however cholesterol concentration was higher. Therefore, altered ganglioside composition differentially affects NKA and LepR abundance, accompanied by lower NKA enzyme activity in the mouse thalamus, suggesting a noteworthy connection between leptin receptor and NKA ion transporter in a diverse glycolipid milieu.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:326334
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-07-04 06:50:38