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Scientific paper - Original scientific paper
Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis
Biomedicines, 11 (2023), 8; 2209. https://doi.org/10.3390/biomedicines11082209
Habek, Mario; Blažeković, Antonela; Gotovac Jerčić, Kristina; Pivac, Nela; Outero, Tiago Fleming; Borovečki, Fran; Brinar, Vesna

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Habek, M., Blažeković, A., Gotovac Jerčić, K., Pivac, N., Outero, T. F., Borovečki, F. & Brinar, V. (2023). Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis. Biomedicines, 11. (8). doi: 10.3390/biomedicines11082209

Habek, Mario, et al. "Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis." Biomedicines, vol. 11, no. 8, 2023. https://doi.org/10.3390/biomedicines11082209

Habek, Mario, Antonela Blažeković, Kristina Gotovac Jerčić, Nela Pivac, Tiago Fleming Outero, Fran Borovečki and Vesna Brinar. "Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis." Biomedicines 11, no. 8 (2023). https://doi.org/10.3390/biomedicines11082209

Habek, M., et al. (2023) 'Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis', Biomedicines, 11(8). doi: 10.3390/biomedicines11082209

Habek M, Blažeković A, Gotovac Jerčić K, Pivac N, Outero TF, Borovečki F, and sur.. Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis. Biomedicines [Internet]. 2023 August 07 [cited 2024 November 10];11(8). doi: 10.3390/biomedicines11082209

M. Habek, et al., "Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis", Biomedicines, vol. 11, no. 8, August 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:519912. [Accessed: 10 November 2024]

Metadata
Title (english)Genome-Wide Expression Profile in People with Optic Neuritis Associated with Multiple Sclerosis
AuthorMario Habek
AuthorAntonela Blažeković
AuthorKristina Gotovac Jerčić
AuthorNela Pivac
AuthorTiago Fleming Outero
AuthorFran Borovečki
AuthorVesna Brinar
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Neurology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Neurology
Abstract (english)
The aim of this study was to perform a genome-wide expression analysis of whole-blood samples from people with optic neuritis (ON) and to determine differentially expressed mRNAs compared to healthy control subjects. The study included eight people with acute ON and six healthy control subjects. Gene expression was analyzed using DNA microarrays for whole-human-genome analysis, which contain 54,675 25-base pairs. The additional biostatistical analysis included gene ontology analysis and gene set enrichment analysis (GSEA). Quantitative RT-PCR (qPCR) was used to confirm selected differentially expressed genes. In total, 722 differently expressed genes were identified, with 377 exhibiting increased, and 345 decreased, expression. Gene ontology analysis and GSEA revealed that protein phosphorylation and intracellular compartment, apoptosis inhibition, pathways involved in cell cycles, T and B cell functions, and anti-inflammatory central nervous system (CNS) pathways are implicated in ON pathology. qPCR confirmed the differential expression of eight selected genes, with SLPI, CR3, and ITGA4 exhibiting statistically significant results. In conclusion, whole-blood gene expression analysis showed significant differences in the expression profiles of people with ON compared to healthy control subjects. Additionally, pathways involved in T cell regulation and anti-inflammatory pathways within CNS were identified as important in the early phases of MS.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBiomedicines
Numberingvol. 11, no. 8, 2209
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines11082209
URN:NBNurn:nbn:hr:105:519912
Publication2023-08-07
Document URLhttps://www.mdpi.com/2227-9059/11/8/2209
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-08-20 10:04:39