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Scientific paper - Original scientific paper
Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke
Journal of Photochemistry and Photobiology B: Biology, 253 (2024); 112871. https://doi.org/10.1016/j.jphotobiol.2024.112871
Josić Dominović, P.; Dobrivojević Radmilović, M.; Srakočić, S.; Mišerić, I.; Škokić, S.; Gajović, Srećko

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Josić Dominović, P., Dobrivojević Radmilović, M., Srakočić, S., Mišerić, I., Škokić, S. & Gajović, S. (2024). Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke. Journal of Photochemistry and Photobiology B: Biology, 253.. doi: 10.1016/j.jphotobiol.2024.112871

Josić Dominović, P., et al. "Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke." Journal of Photochemistry and Photobiology B: Biology, vol. 253, 2024. https://doi.org/10.1016/j.jphotobiol.2024.112871

Josić Dominović, P., M. Dobrivojević Radmilović, S. Srakočić, I. Mišerić, S. Škokić and Srećko Gajović. "Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke." Journal of Photochemistry and Photobiology B: Biology 253 (2024). https://doi.org/10.1016/j.jphotobiol.2024.112871

Josić Dominović, P., et al. (2024) 'Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke', Journal of Photochemistry and Photobiology B: Biology, 253. doi: 10.1016/j.jphotobiol.2024.112871

Josić Dominović P, Dobrivojević Radmilović M, Srakočić S, Mišerić I, Škokić S, Gajović S. Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke. Journal of Photochemistry and Photobiology B: Biology [Internet]. 2024 April [cited 2024 November 22];253. doi: 10.1016/j.jphotobiol.2024.112871

P. Josić Dominović, M. Dobrivojević Radmilović, S. Srakočić, I. Mišerić, S. Škokić and S. Gajović, "Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke", Journal of Photochemistry and Photobiology B: Biology, vol. 253, April 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:271637. [Accessed: 22 November 2024]

Metadata
Title (english)Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke
AuthorP. Josić Dominović
AuthorM. Dobrivojević Radmilović
AuthorS. Srakočić
AuthorI. Mišerić
AuthorS. Škokić
AuthorSrećko Gajović
Author's institutionUniversity of Zagreb
School of Medicine
(Croatian Institiute for Brain Research)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
Programmed cell death or apoptosis is a critically important mechanism of tissue remodeling and regulates conditions such as cancer, neurodegeneration or stroke. The aim of this research article was to assess the caged Z-DEVD-aminoluciferin substrate for in vivo monitoring of apoptosis after ischemic stroke in TLR2-deficient mice and their TLR2-expressing counterparts. Postischemic inflammation is a significant contributor to ischemic injury development and apoptosis, and it is modified by the TLR2 receptor. Caged Z-DEVD-aminoluciferin is made available for bioluminescence enzymatic reaction by cleavage with activated caspase-3, and therefore it is assumed to be capable of reporting and measuring apoptosis. Apoptosis was investigated for 28 days after stroke in mice which ubiquitously expressed the firefly luciferase transgene. Middle cerebral artery occlusion was performed to achieve ischemic injury, which was followed with magnetic resonance imaging. The scope of apoptosis was determined by bioluminescence with caged Z-DEVD-aminoluciferin, immunofluorescence with activated caspase-3, flow cytometry with annexin-V and TUNEL assay. The linearity of Z-DEVD-aminoluciferin substrate dose effect was shown in the murine brain. Z-DEVD-aminoluciferin was validated as a good tool for monitoring apoptosis following adequate adjustment. By utilizing bioluminescence of Z-DEVD-aminoluciferin after ischemic stroke it was shown that TLR2-deficient mice had lower post-stroke apoptosis than TLR2-expressing wild type mice. In conclusion, Z-DEVD-aminoluciferin could be a valuable tool for apoptosis measurement in living mice.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleJournal of Photochemistry and Photobiology B: Biology
Numberingvol. 253, 112871
p-ISSN1011-1344
DOIhttps://doi.org/10.1016/j.jphotobiol.2024.112871
URN:NBNurn:nbn:hr:105:271637
Publication2024-04
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S1011134424000319
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-11-11 09:21:23