prikaz prve stranice dokumenta Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?
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Scientific paper - Review paper
Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?
Biomolecules, 14 (2024), 2; 156. https://doi.org/10.3390/biom14020156
Mlinac-Jerković, Kristina; Kalanj-Bognar, Svjetlana; Heffer, Marija; Blažetić, Senka

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Mlinac Jerković, K., Kalanj Bognar, S., Heffer, M. & Blažetić, S. (2024). Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?. Biomolecules, 14. (2). doi: 10.3390/biom14020156

Mlinac Jerković, Kristina, et al. "Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?." Biomolecules, vol. 14, no. 2, 2024. https://doi.org/10.3390/biom14020156

Mlinac Jerković, Kristina, Svjetlana Kalanj Bognar, Marija Heffer and Senka Blažetić. "Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?." Biomolecules 14, no. 2 (2024). https://doi.org/10.3390/biom14020156

Mlinac Jerković, K., et al. (2024) 'Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?', Biomolecules, 14(2). doi: 10.3390/biom14020156

Mlinac Jerković K, Kalanj Bognar S, Heffer M, Blažetić S. Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?. Biomolecules [Internet]. 2024 January 28 [cited 2024 November 22];14(2). doi: 10.3390/biom14020156

K. Mlinac Jerković, S. Kalanj Bognar, M. Heffer and S. Blažetić, "Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?", Biomolecules, vol. 14, no. 2, January 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:213449. [Accessed: 22 November 2024]

Metadata
Title (english)Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?
AuthorKristina Mlinac-Jerković
AuthorSvjetlana Kalanj-Bognar
AuthorMarija Heffer
AuthorSenka Blažetić
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Chemistry, Biochemistry and Clinical Chemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The purpose of this review is to succinctly examine the methodologies used in lipid raft research in the brain and to highlight the drawbacks of some investigative approaches. Lipid rafts are biochemically and biophysically different from the bulk membrane. A specific lipid environment within membrane domains provides a harbor for distinct raftophilic proteins, all of which in concert create a specialized platform orchestrating various cellular processes. Studying lipid rafts has proved to be arduous due to their elusive nature, mobility, and constant dynamic reorganization to meet the cellular needs. Studying neuronal lipid rafts is particularly cumbersome due to the immensely complex regional molecular architecture of the central nervous system. Biochemical fractionation, performed with or without detergents, is still the most widely used method to isolate lipid rafts. However, the differences in solubilization when various detergents are used has exposed a dire need to find more reliable methods to study particular rafts. Biochemical methods need to be complemented with other approaches such as live-cell microscopy, imaging mass spectrometry, and the development of specific non-invasive fluorescent probes to obtain a more complete image of raft dynamics and to study the spatio-temporal expression of rafts in live cells.
Keywords (english)
Languageenglish
Publication typeScientific paper - Review paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBiomolecules
Numberingvol. 14, no. 2, 156
e-ISSN2218-273X
DOIhttps://doi.org/10.3390/biom14020156
URN:NBNurn:nbn:hr:105:213449
Publication2024-01-28
ProjectNumber: IP-2016-06-8636
Title (croatian): Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga
Title (english): Molecular markers of neuronal vulnerability, adaptation and plasticity in acute and chronic brain lesion
Acronym: NeuroReact
Leader: Svjetlana Kalanj-Bognar
Jurisdiction: Croatia
Funding stream: Research Projects
ProjectNumber: IP-2014-09-2324
Title (croatian): Patofiziološke posljedice promjena sastava lipidnih splavi
Title (english): Pathophysiological consequences of lipid raft changes
Acronym: RafTuning
Leader: Marija Heffer
Jurisdiction: Croatia
Funding stream: Research Projects
Document URLhttps://www.mdpi.com/2218-273X/14/2/156
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-11-15 10:56:15