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doctoral thesis
Molecular diversity among adult hippocampal and entorhinal cells
Zagreb: University of Zagreb, School of Medicine, 2025. urn:nbn:hr:105:053196
Franjić, Daniel
University of Zagreb
School of Medicine
Croatian Institiute for Brain Research

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Franjić, D. (2025). Molecular diversity among adult hippocampal and entorhinal cells (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:053196

Franjić, Daniel. "Molecular diversity among adult hippocampal and entorhinal cells." Doctoral thesis, University of Zagreb, School of Medicine, 2025. https://urn.nsk.hr/urn:nbn:hr:105:053196

Franjić, Daniel. "Molecular diversity among adult hippocampal and entorhinal cells." Doctoral thesis, University of Zagreb, School of Medicine, 2025. https://urn.nsk.hr/urn:nbn:hr:105:053196

Franjić, D. (2025). 'Molecular diversity among adult hippocampal and entorhinal cells', Doctoral thesis, University of Zagreb, School of Medicine, accessed 17 March 2025, https://urn.nsk.hr/urn:nbn:hr:105:053196

Franjić D. Molecular diversity among adult hippocampal and entorhinal cells [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2025 [cited 2025 March 17] Available at: https://urn.nsk.hr/urn:nbn:hr:105:053196

D. Franjić, "Molecular diversity among adult hippocampal and entorhinal cells", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2025. Available at: https://urn.nsk.hr/urn:nbn:hr:105:053196

Metadata
TitleMolecular diversity among adult hippocampal and entorhinal cells
Title (english)Molecular diversity among adult hippocampal and entorhinal cells
AuthorDaniel Franjić
MentorGoran Sedmak (mentor)
MentorNenad Šestan (komentor)
VIAF: 316026737
Committee memberGoran Šimić (predsjednik povjerenstva)
Committee memberMario Vukšić (član povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Croatian Institiute for Brain Research)
Zagreb
Defense date and country2025-01-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
The hippocampal system is a critical component in cognition, adult neurogenesis, and selectively sensitive to aging and neurodegenerative processes. To gain insights into neurogenic potential and diversity of cell types in adult humans, we profiled single-nucleus transcriptomes in five hippocampal subregions. Integration of dentate gyrus data with mouse, pig and macaque, showed strong neurogenesis trajectories in these species that was absent in humans. Doublecortin (DCX), the common marker of neurogenesis in the dentate gyrus, showed widespread expression in the human hippocampal system, including some mature dentate granule cells, but did not define immature cell populations in humans. We further revealed prominent diversity among excitatory neurons across the hippocampal system that echoed the transition from three-layered archicortex to six-layered neocortex. Our results highlighted the uncharacterized METTL7B associated with Alzheimer’s disease-related proteins and enriched in primates in neuronal subtypes and subregions more resilient to early neuropathological changes in the Alzheimer’s disease. Overall, our findings unveiled cell typespecific molecular characteristics pertinent to hippocampal physiological function.
Abstract (croatian)
Neuralni krugovi hipokampalne (HIP) i entorinalne moždane kore (EC) ključni su dijelovi široke neuronske mreže odgovorne za pamćenje te reprezentaciju prostora i vremena. U svrhu boljeg uvida u pojedinačne neuronske i ne-neuronske populacije tog sustava, provedeno je RNA sekvencioniranje pojedinačnih staničnih jezgara (snRNA-seq) uzoraka iz pet anatomski definiranih podregija hipokampalnog sustava. Integrirana analiza između vrsta pokazala je transkriptomske i histološke znakove neurogeneze u odraslim miševima, svinjama i makaki majmunima, ali ne i u ljudi. DCX, marker novonastalih zrnatih stanica, pronađen je u različitim neuronskim populacijama odraslog čovjeka, ali nije definirao populacije nezrelih neurona. Također, opisali smo specifične, subregionalno transkriptomski definirane tipove stanica i promjene u prijelazu iz troslojnog arhikorteksa u šestoslojni neokorteks. Naši rezultati istaknuli su da je METTL7B povezan s proteinima povezanim s Alzheimerovom bolešću i obogaćen u primata u podtipovima stanica i podregijama otpornijim na rane neuropatološke promjene u Alzheimerovoj bolesti. Ovo istraživanje je otkrilo molekularne karakteristike određenih vrsta i podtipova stanica koje potencijalno imaju važnu ulogu u fiziološkoj funkciji i/ili patološkim promjenama hipokampalnog sustava.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:105:053196
Study programmeTitle: Neuroscience
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent159 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-01-24 09:23:40