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master's thesis
Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije
Zagreb: University of Zagreb, School of Medicine, 2016. urn:nbn:hr:105:749728
Rašić, Mario
University of Zagreb
School of Medicine
Department of Physics and Biophysics

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Rašić, M. (2016). Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:749728

Rašić, Mario. "Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije." Master's thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:749728

Rašić, Mario. "Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije." Master's thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:749728

Rašić, M. (2016). 'Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije', Master's thesis, University of Zagreb, School of Medicine, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:749728

Rašić M. Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2016 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:105:749728

M. Rašić, "Praćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:105:749728

Metadata
TitlePraćenje utjecaja valproata na molekularni sastav medija tijekom kulture štakorskog zametka metodom infracrvene spektrometrije
Title (english)Monitoring of valproate effect on the molecular composition of the medium during rat embryo culture assessed by infrared spectroscopy
AuthorMario Rašić
MentorMaja Balarin (mentor)
Committee memberOzren Gamulin (predsjednik povjerenstva)
Committee memberSanja Dolanski-Babić (član povjerenstva)
Committee memberMaja Balarin (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Physics and Biophysics)
Zagreb
Defense date and country2016-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uzorci medija u kojima su rasli štakorski zameci analizirani su FTIR spektroskopijom. Dobiveni spektri su uprosječeni, obrađeni pomoću Studentovog t-testa i multivarijatnom statističkom metodom analize principalne komponente (PCA). Pokazano je da se FTIR spektroskopijom može proučavati biološki materijal odnosno medij u kojem je rastao i razvijao se štakorski zametak. Također je ustvrđeno da se pomoću FTIR spektroskopije može pratiti utjecaj teratogenih supstanci poput valproata koji svojim učinkom mijenja embrij, a samim time i molekularni sastav medija u kojem je zametak rastao. Taj molekularni sastav je moguće pratiti kroz dane razvoja štakorskog zametka. Analiza uprosječenih spektara pokazala je da je utjecaj valproata najveći tijekom neurulacije štakorskog zametka, odnosno da je molekularni sastav medija kontrolne skupine i skupine s valproatom tada najrazličitiji. Pored toga, prikazano je kako je utjecaj valproata na molekularni sastav medija najmanji tijekom četrnaestog dana kulture štakorskog zametka, kada je on na samom kraju svog razvitka prije nego što postane fetus. Rezultati ovog diplomskog ukazuju da je FTIR spektroskopija izuzetno oruđe u proučavanju i praćenu utjecaja valproata na molekularni sastava medija tijekom kulture štakorskog zametka.
Abstract (english)
Samples of the rat embryo media in which they grew were analyzed by FTIR spectroscopy. The resulting spectra were averaged and analyzed using Student's t-test and multivariate statistical method, principal component analysis (PCA). It is shown that FTIR spectroscopy can be used to study the biological material such as the medium during rat embryo culture. It is also detected that by using FTIR spectroscopy we can monitor the impact of teratogenic substances such as valproic acid on rat embryos, which by its effect changes the embryo, and therefore the molecular composition of the medium during rat embryo culture. The molecular composition of medium can be followed through the days of the development of rat embryos. The analyzis of averaged spectra revealed that the valproic acid has the highest impact during neurulation of the rat embryo. At that period the molecular composition of the medium in control group and the group treated with valproate is the most different. In addition, it is shown how the impact of the valproate on the molecular composition of the medium is the least significant during the fourteenth day of rat embryo culture when it is in the final stage of its development, before it becomes a fetus. The results of this diploma thesis show that FTIR spectroscopy is an exceptional tool for observing and following the impact of valproate on the molecular composition of the medium during rat embryo culture.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:749728
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-07-11 12:09:48