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master's thesis
Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima
Zagreb: University of Zagreb, School of Medicine, 2016. urn:nbn:hr:105:669602
Marković, Leon
University of Zagreb
School of Medicine
Department of Medical Biology

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Marković, L. (2016). Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:669602

Marković, Leon. "Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima." Master's thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:669602

Marković, Leon. "Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima." Master's thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:669602

Marković, L. (2016). 'Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima', Master's thesis, University of Zagreb, School of Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:669602

Marković L. Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2016 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:105:669602

L. Marković, "Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:105:669602

Metadata
TitleČimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima
Title (english)Transcription factors TCF-1 and LEF-1 involved in Wnt signaling in astrocyte brain tumors
AuthorLeon Marković
MentorNives Pećina-Šlaus (mentor)
Committee memberDavor Tomas (predsjednik povjerenstva)
Committee memberLjiljana Šerman (član povjerenstva)
Committee memberNives Pećina-Šlaus (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Zagreb
Defense date and country2016-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Neurology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Oncology
Abstract
Obitelj transkripcijskih faktora TCF/LEF od velikog je značaja u biologiji tumora. Predmet istraživanja u ovom radu bila je razina ekspresije TCF-1 i LEF-1, u astrocitomima različitih histopatoloških gradusa (I, II i III). Razine ekspresije TCF-1 i LEF-1 određene su metodom imunohistokemije. Utvrđena je ekspresija transkripcijskih čimbenika TCF-1 i LEF-1 lokalizirana u jezgrama stanica astrocitoma. Za TCF-1 određen je porast udjela stanica u kategorijama jake i srednje razine ekspresije, koji je u pozitivnoj korelaciji sa porastom gradusa, sa najvišim vrijednostima u astrocitomima gradusa III -jaka (4,67%), srednja (51,85%), a u njima je očekivano smanjen udio stanica niske ekspresije (43,46%). Za LEF-1 utvrđen je pad ekspresije u kategorijama srednje i niske razine ekspresije, dok je za razinu jake ekspresije određen porast ekspresije koji korelira sa porastom gradusa astrocitoma, sa najvišom vrijednošću jake ekspresije u astrocitomima III (3,73%). Prema našim rezultatima zaključujemo da je porast ekspresije TCF-1 i LEF-1 u pozitivnoj korelaciji sa porastom gradusa astrocitoma. Izraženost transkripcijskih čimbenika TCF-1 i LEF-1 upućuje na uključenost signalnog puta Wnt u nastanku i progresiji tumora mozga astrocitoma.
Abstract (english)
TCF/LEF family of transcription factors is of great significance in tumor biology. The subject of this research was to determine the levels of expression of TCF-1 and LEF-1, in astrocytoma of different histopathologic grade (I, II and III). Immunohistochemistry was the method of choice for determining the levels of expression of TCF-1 and LEF-1. The expression of transcription factors TCF-1 and LEF-1 was localised within the nuclei of astrocytic tumor cells. In case of TCF-1, there was an increase in percentage of cells in categories of strong and intermediate levels of expression, which is in positive correlation with the increasement of grade, with peak values in astrocytoma grade III -strong (4,67%), intermediate (51,85%), as expected there was decreasement in percentage of cells with low expression (43,46%). On the other hand, results for LEF-1 displayed decreased expression in categories with intermediate and low levels of expression, while for the level of strong expression was determined increase in expression which correlated with the increasement of astrocytomic grade, with the peak value in astrocytoma III (3,73). Based on our results, we conclude that the increase in expression of TCF-1 and LEF-1 is in positive correlation with the increase in astrocytoma grade. The expression of transcription factors TCF-1 and LEF-1 suggests activation of Wnt signaling pathway in formation and progression of astrocytic brain tumors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:669602
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-08-21 09:02:43