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master's thesis
Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda
Zagreb: University of Zagreb, School of Medicine, 2017. urn:nbn:hr:105:816883
Slatina, Paula
University of Zagreb
School of Medicine
Department of Medical Biology

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Slatina, P. (2017). Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:816883

Slatina, Paula. "Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda." Master's thesis, University of Zagreb, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:105:816883

Slatina, Paula. "Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda." Master's thesis, University of Zagreb, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:105:816883

Slatina, P. (2017). 'Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda', Master's thesis, University of Zagreb, School of Medicine, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:816883

Slatina P. Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2017 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:105:816883

P. Slatina, "Izraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:105:816883

Metadata
TitleIzraženost proteina obitelji Dishevelled u posteljicama kod zastoja u rastu ploda
Title (english)Expression of dishevelled protein family in the placenta in intrauterine growth restriction
AuthorPaula Slatina
MentorLjiljana Šerman (mentor)
Committee memberRatko Matijević (predsjednik povjerenstva)
Committee memberTamara Nikuševa Martić (član povjerenstva)
Committee memberLjiljana Šerman (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Zagreb
Defense date and country2017-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Wnt signalni put igra važnu ulogu u razvoju placente i diferencijaciji ljudskog trofoblasta, a Dishevelled proteini su njegovi centralni medijatori koji omogućuju finu regulaciju aktivnosti ovog signalnog puta. Prema dosadašnjim spoznajama neuspješna invazija trofoblasta zapažena je u posteljicama fetusa sa zastojem u rastu (IUGR) i u hipertenzivnim poremećajima. Budući da Dishevelled proteini djeluju kao pozitivni regulatori Wnt signalnog puta, cilj ovog rada bio je ispitati ekspresiju DVL1, DVL2, DVL3 proteina u terminskim posteljicama sa zastojem u rastu (IUGR). Analizirane su ukupno 24 terminske posteljice od kojih je 15 bilo iz trudnoća sa zastojem u rastu, a 9 iz urednih trudnoća koje su služile kao kontrola. Napravljena je imunohistokemijska analiza sva tri antigena, a rezultati su kvantificirani stereološkom varijablom volumenske gustoće (Vv, mm0). Statistički značajno manja ekspresija proteina DVL1, DVL2 I DVL3 detektirana je u terminskim posteljicama sa zastojem u rastu ploda u odnosu na zdrave terminske posteljice (p<0,05; Mann Whitney) . Dobiveni rezultati ukazuju na važnost porodice Dishevelled proteina u razvoju IUGR-a te podržavaju hipotezu plitke invazije u nastanku ove patologije trudnoće.
Abstract (english)
Wnt signaling pathway plays an important role in the development of the placenta and the differentiaton of trophoblast. Dishevelled proteins are the central mediators in this pathway which enable its fine regulation. According to previous knowledge, unsuccessfull trophoblast invasion has been noticed in placentas of fetuses with intrauterine growth restriction (IUGR) and hypertensive disorders. Since Dishevelled proteins act as positive regulators of the Wnt signaling pathway, the aim of this study was to examine the expression of DVL1, DVL2 and DVL3 proteins in term placentas with IUGR. 24 term placentas have been analysed of which 15 were those from pregnancies with IUGR and 9 from normal pregnancies which served as a controle. Immunohistochemical analysis was done on all three antigens and the results stereologically quantified using a variable for volume density (Vv, mm0). Statistically significant lower expression of proteins DVL1, DVL2 and DVL3 was detected in term placentas with IUGR in comparison to healthy term placentas (p<0,05; Mann Whitney). The results show the importance of Dishevelled proteins in the formation of IUGR and support the hypothesis of shallow invasion being a factor in the pathogenesis of this condition
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:816883
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-07 11:03:26