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master's thesis
Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša
Zagreb: University of Zagreb, School of Medicine, 2017. urn:nbn:hr:105:621605
Banovac, Ivan
University of Zagreb
School of Medicine
Department of Anatomy and Clinical Anatomy

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Banovac, I. (2017). Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:621605

Banovac, Ivan. "Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša." Master's thesis, University of Zagreb, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:105:621605

Banovac, Ivan. "Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša." Master's thesis, University of Zagreb, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:105:621605

Banovac, I. (2017). 'Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša', Master's thesis, University of Zagreb, School of Medicine, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:621605

Banovac I. Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2017 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:105:621605

I. Banovac, "Utjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:105:621605

Metadata
TitleUtjecaj gena za BMP3 na trabekularne i kortikalne parametre dugih kostiju miša
Title (english)The influence of the BMP3 gene on trabecular and cortical bone parameters of long bones in mice
AuthorIvan Banovac
MentorLovorka Grgurević (mentor)
Committee memberSlobodan Vukičević (predsjednik povjerenstva)
Committee memberZdravko Petanjek (član povjerenstva)
Committee memberLovorka Grgurević (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Anatomy and Clinical Anatomy)
Zagreb
Defense date and country2017-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Anatomy
Abstract
BMP3 najučestaliji je BMP izoliran iz demineralizirane kosti i djeluje in vivo kao antagonist ostalih osteoinduktivnih BMP-ova. Istraživanje je provedeno na miševima s izbačenim genom za BMP3 oba spola, 2 i 4 mjeseca starosti. Miševi su genotipizirani i podijeljeni u skupine prema genotipu (WT i KO), dobi i spolu. Kosti miševa snimljene su ex vivo pomoću SkyScan 1076 mikro CT uređaja te je napravljena morfometrijska analiza trabekularnih i kortikalnih parametara distalnog femura i proksimalne tibije. Morfometrijskom analizom utvrđeno je da KO miševi imaju veći volumen kostiju (BV/TV) od WT miševa. Povećani BV/TV ustanovljen je u miševa obje dobne skupine i oba spola. Analizom je također utvrđeno da je povećanje koštanog volumena kortikalne kosti i debljine kortikalne kosti (Co.Th) kod KO životinja posljedica smanjenjene endostealne resorpcije pokazano jakom korelacijom endostealnog volumena (EV) s BV/TV. Povećanje koštanog volumena trabekularne kosti kod KO životinja posljedica je povećanja broja trabekula (Tb.N) i smanjenja razmaka između trabekula (Tb.Sp.) pokazano jakom korelacijom s BV/TV. Nije utvrđena značajna razlika u debljini trabekula (Tb.Th.) između KO i WT životinja što je potvrđeno slabom korelacijom s BV/TV.
Abstract (english)
BMP3 is the most abundant BMP in demineralised bone and antagonises other osteoinductive BMPs in vivo. The research was carried out on BMP3 knock-out mice of both sexes aged 2 and 4 months. After genotyping, the mice were divided into groups according to genotype (WT and KO), age and sex. The bones of the mice were recorded ex vivo using a SkyScan 1076 micro CT device and a subsequent morphometric analysis of trabecular and cortical parameters of the distal femur and proximal tibia was carried out. The morphometric analysis established that KO mice have higher bone volume (BV/TV) than WT mice. An increase in BV/TV was found in all groups of mice. The analysis also found that the increases in bone volume of cortical bone and cortical thickness (Co.Th) in KO mice were due to reduced endosteal resorption shown by a strong correlation between endosteal volume (EV) and BV/TV. The increase in bone volume of trabecular bone in KO mice was due to increased trabecular number (Tb.N) and decreased trabecular separation (Tb.Sp) shown by a strong correlation with BV/TV. No significant difference in trabecular thickness (Tb.Th) was found between KO and WT mice, confirmed by a weak correlation with BV/TV.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:621605
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteRad je nagrađen Rektorovom nagradom u akademskoj godini 2015./2016.
Created on2017-11-13 07:47:35