Discovery of new markers for diagnosis of Alzheimer's disease (AD) is of great importance in terms of early diagnosis of the disease and possibly finding an appropriate cure for the disease. Currently, diagnosis of AD is confirmed only in the latter stages of the disease when possibilities of preventive strategies that may slow down progression of the disease are minimal. AD is mostly diagnosed by clinical criteria, but in recent years ancillary markers are increasingly used to improve sensitivity and specificity of diagnosis (e.g. cerebrospinal fluid markers, MRI and PET markers). It is well known that the pathological process begins more than a decade before first symptoms onset, which is the main focus of early diagnosis of AD. This paper discusses biochemical markers that are currently in everyday clinical use as well as potential promising candidates. Thereby, discovery of specific and sensitive markers, especially the ones found in the blood, could be regarded as a breakthrough in earlier and more accurate diagnosis of AD. Introductory part of this paper contains main facts about AD, diagnostic and management protocols in AD, while second part discusses currently known biochemical markers (from cerebrospinal fluid and blood) that are either already in use or have great perspective in the future of diagnostics and are currently used in research purposes. In third part of this paper, conclusion is given with future perspectives of biological markers in diagnosis of AD.