Hospital-acquired infections present a major burden and a challenge facing modern health system that directly endanger the patient's safety and affect healthcare service quality. In the future, the problem of these infections is expected to be considerably larger and more complex. Considering the fact that healthcare-associated infections are a global problem, there is also a need for involvement of as many countries of the world as possible inmutual long-term and multidisciplinary cooperation in monitoring and controlling the healthcare- associated infections. The most important means of limiting their occurrence and spread isan expert and well-organized infection control system. Aim of the paper: The aim of the study is to evaluate the burden (prevalence) of hospital-acquired infections and antimicrobial drugs prescribing in General Hospital Varaždin. Another aim was to identify the most common types of infections, types and indications for the application of antimicrobial drugs, prophylactic application of antibiotics in the hospital, patients, procedures, microorganisms (agents). Respondents and Methods: GH Varaždin participated in an International Prevalence Study on Infections Related to Health Care and Antibiotic Use in May 2017. In the stated period, 251 patients who met the criteria of the study were included. Data was collected by standard form selection issued by ECDC, which was later enrolled in the electronic HelicsWin.Net form. Conclusion: The results of this paper confirmed that the rate ofhospital-acquired infectionsin GH Varaždin is within the average of the Republic of Croatia and lower than the European average of the PPS study conducted in 2011-2012 and that the consumption of antimicrobial medicines is within the Croatian average.