prikaz prve stranice dokumenta Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice
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Scientific paper - Original scientific paper
Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice
International Journal of Molecular Sciences, 20 (2019), 20; 5188. https://doi.org/10.3390/ijms20205188
Kozina, Nataša; Mihaljević, Zrinka; Baus Lončar, Mirela; Mihalj, Martina; Mišir, Mihael; Radmilović, Marina; Justić, Helena; Gajović, Srećko; Šešelja, Kate; Bazina, Iva; Horvatić, Anita; Matić, Anita; Bijelić, Nikola; Rođak, Edi; Jukić, Ivana; Drenjančević, Ines More authors...

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Kozina, N., Mihaljević, Z., Baus Lončar, M., Mihalj, M., Mišir, M., Radmilović, M. ... Drenjančević, I. (2019). Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice. International Journal of Molecular Sciences, 20. (20). doi: 10.3390/ijms20205188

Kozina, Nataša, et al. "Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice." International Journal of Molecular Sciences, vol. 20, no. 20, 2019. https://doi.org/10.3390/ijms20205188

Kozina, Nataša, Zrinka Mihaljević, Mirela Baus Lončar, Martina Mihalj, Mihael Mišir, Marina Radmilović, Helena Justić, et al. "Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice." International Journal of Molecular Sciences 20, no. 20 (2019). https://doi.org/10.3390/ijms20205188

Kozina, N., et al. (2019) 'Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice', International Journal of Molecular Sciences, 20(20). doi: 10.3390/ijms20205188

Kozina N, Mihaljević Z, Baus Lončar M, Mihalj M, Mišir M, Radmilović M, and sur.. Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice. International Journal of Molecular Sciences [Internet]. 2019 October 19 [cited 2024 November 22];20(20). doi: 10.3390/ijms20205188

N. Kozina, et al., "Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice", International Journal of Molecular Sciences, vol. 20, no. 20, October 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:792149. [Accessed: 22 November 2024]

Metadata
Title (english)Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3−/−/C57BL/6N Knockout and WT (C57BL/6N) Control Mice
AuthorNataša Kozina
AuthorZrinka Mihaljević
AuthorMirela Baus Lončar
AuthorMartina Mihalj
AuthorMihael Mišir
AuthorMarina Radmilović
AuthorHelena Justić
AuthorSrećko Gajović
AuthorKate Šešelja
AuthorIva Bazina
AuthorAnita Horvatić
AuthorAnita Matić
AuthorNikola Bijelić
AuthorEdi Rođak
AuthorIvana Jukić
AuthorInes Drenjančević
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3−/− gene knockout mice (Tff3−/−/C57BL/6N) have changes in lipid metabolism which may affect vascular function and outcomes of stroke. We aimed to study the effects of one week of HS diet (4% NaCl) on vascular function and stroke induced by transient occlusion of middle cerebral artery in Tff3−/− and wild type (WT/C57BL/6N) mice. Flow-induced dilation (FID) of carotid artery was reduced in WT-HS mice, but not affected in Tff3−/−-HS mice. Nitric oxide (NO) mediated FID. NO production was decreased with HS diet. On the contrary, acetylcholine-induced dilation was significantly decreased in Tff3−/− mice on both diets and WT-HS mice. HS intake and Tff3 gene depletion affected the structural components of the vessels. Proteomic analysis revealed a significant effect of Tff3 gene deficiency on HS diet-induced changes in neuronal structural proteins and acute innate immune response proteins’ expression and Tff3 depletion, but HS diet did not increase the stroke volume, which is related to proteome modification and upregulation of genes involved mainly in cellular antioxidative defense. In conclusion, Tff3 depletion seems to partially impair vascular function and worsen the outcomes of stroke, which is moderately affected by HS diet.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 20, no. 20, 5188
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms20205188
URN:NBNurn:nbn:hr:105:792149
Publication2019-10-19
Document URLhttps://www.mdpi.com/1422-0067/20/20/5188
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-02-23 12:03:00