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doctoral thesis
Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti
Zagreb: University of Zagreb, School of Medicine, 2020. urn:nbn:hr:105:960211
Lončar, Andrija
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Lončar, A. (2020). Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:960211

Lončar, Andrija. "Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti." Doctoral thesis, University of Zagreb, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:105:960211

Lončar, Andrija. "Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti." Doctoral thesis, University of Zagreb, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:105:960211

Lončar, A. (2020). 'Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti', Doctoral thesis, University of Zagreb, School of Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:960211

Lončar A. Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2020 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:105:960211

A. Lončar, "Rane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:105:960211

Metadata
TitleRane promjene u mozgu izazvane središnjom primjenom streptozotocina u eksperimentalnom modelu sporadične Alzheimerove bolesti
Title (english)Early changes in the brain induced by central administration of streptozotocin in the experimental model of sporadic Alzheimer's disease
AuthorAndrija Lončar
VIAF: 18161096286229100382
MentorMelita Šalković-Petrišić (mentor)
Committee memberGoran Šimić (predsjednik povjerenstva)
Committee memberNataša Jovanov Milošević (član povjerenstva)
Committee memberSvjetlana Kalanj-Bognar (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2020-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Universal decimal classification
(UDC)		615 - Pharmacology. Therapeutics. Toxicology
Abstract
Alzheimerova bolest (AB) je neizlječiva neurodegenerativna bolest koju klinički obilježava oštećenje kognicije i ponašanja, a neuropatološki nakupljanje senilnih plakova, neurofibrilarnih snopića i gubitak neurona. Najčešći je sporadičan oblik bolesti (sAB) s dugim pretkliničkim tijekom i nepoznatim uzrokom. Intracerebroventrikularni streptozotocinski model (STZ-icv) jedan je od netransgeničnih životinjskih modela koji oponašaju humanu sAB. Cilj istraživanja je bio ispitati rane promjene unutar 24h u mozgu miša i štakora, na razini signalnog puta inzulisnkog receptora, fosforilacije proteina tau, kolinergičnog sustava te morfoloških i strukturnih promjena. Promjene načelno pokazuju regionalnu specifičnost izraženiju u parijetalnom korteksu (PC), pogotovo u mišjem modelu, i vremensku specifičnost - svi mjereni parametri promijenjeni su 1h po STZ-icv primjeni, najizraženije nakon 24h. Ekspresija inzulinskog receptora (IR) i nosača za glukozu 2 (GLUT2) upućuje na negativnu korelaciju, jasnije izražnu u štakorskom modelu koji je i osjetljiviji na nastanak tau patologije od mišjeg. Aktivnost acetilkolinesteraze (AChE) je izraženija u PC-u. Astroglioza se javlja 1h po STZ-icv i izraženija je u hipokampusu (HPC), dok morfološke promjene nisu zabilježene. Mozak štakora manje je osjetljiv na toksičnost STZ-icv od mišjeg te bolje oponaša humane promjene signalnog puta IR u sAB-u. Između ova dva STZ-icv modela postoje razlike koje ne podupiru alternativnu uporabu ovih modela kao istog, zbog čega su potrebna dodatna komparativna istraživanja.
Abstract (english)
Sporadic Alzheimer's disease (AD) is the most common cause of dementia, which has a long presymptomatic stage and is of unknown cause. Main characteristics of sAD are cognitive disfunction, impaired cholinergic transmission, accumulation of senile plaques, neurofibrillary tangles and loss of neurons. We aimed to explore early alterations within 24h in insulin receptor signaling pathway, phosphorylation of tau proteins, cholinergic system, morphological and structural changes in rats and mice treated intracerebroventricularly with streptozotocin (STZ-icv), proposed as a representative sAD model. All measured parameters were altered 1h after STZ-icv, but most pronounced changes were found after 24 hours. Changes were also region-specific and more pronounced in the parietal cortex (PC) than in the hippocampus (HPC), being more clearly expressed in the mice model. The expression of insulin receptor (IR) and glucose transporter 2 (GLUT2) is showing a negative correlation. Rat seems to be more sensitive to hyperphosphorylation of tau protein than the mice. Acetylcholinesterase (AChE) activity is more elevated in PC. Astrogliosis is most pronounced in HPC and occurs 1h post STZ-icv. The rat brain seems to be less sensitive to STZ-icv pathology than the murine one and mimics changes in the human brain IR signaling pathway better. Observed differences do not support the alternative use of these two rodent models as a one model for sAD.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:960211
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Catalog URLhttps://katalog.nsk.hr/F/19IT7Y755V1NXQJPHCLYSQGPF6BLAPPF179HQ7FAXXMYBYGMV5-44403?func=find-e&request=Rane+promjene+u+mozgu+izazvane+sredi%C5%A1njom+primjenom+streptozotocina+u+eksperimentalnom+modelu+sporadi%C4%8Dne+Alzheimerove+bolesti&find_scan_code=FIND_NAS&adjacent=N&local_base=MF_WEB&x=0&y=0&filter_code_1=WLN&filter_request_1=&filter_code_2=WYR&filter_request_2=&filter_code_3=WYR&filter_request_3=&filter_code_4=WFM&filter_request_4=
Type of resourceText
Extent107 str.
File originBorn digital
Access conditionsOpen access
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Created on2021-03-16 11:44:20