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doctoral thesis
Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom
Zagreb: University of Zagreb, School of Medicine, 2020. urn:nbn:hr:105:875771
Jurakić Tončić, Ružica
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Jurakić Tončić, R. (2020). Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:875771

Jurakić Tončić, Ružica. "Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom." Doctoral thesis, University of Zagreb, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:105:875771

Jurakić Tončić, Ružica. "Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom." Doctoral thesis, University of Zagreb, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:105:875771

Jurakić Tončić, R. (2020). 'Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom', Doctoral thesis, University of Zagreb, School of Medicine, accessed 29 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:875771

Jurakić Tončić R. Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2020 [cited 2024 December 29] Available at: https://urn.nsk.hr/urn:nbn:hr:105:875771

R. Jurakić Tončić, "Određivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:105:875771

Metadata
TitleOdređivanje genotipskih i fenotipskih biomarkera kožne barijere u bolesnika s atopijskim dermatitisom
Title (english)Determination of genotypic and fenotypic biomarkers of skin barrier in skin of patients with atopic dermatitis
AuthorRužica Jurakić Tončić
MentorBranka Marinović (mentor)
MentorSanja Kežić (komentor)
VIAF: 305471531
Committee memberLiborija Lugović Mihić (predsjednik povjerenstva)
VIAF: 305540496
Committee memberAsija Stipić-Marković (član povjerenstva)
Committee memberMarija Buljan (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2020-10-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Dermatovenerology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
U ovoj studiji određivani su genotipski i fenotipski biomarkeri kožne barijere kod bolesnika s atopijskim dermatitisom (AD). U studiju je uključeno 100 odraslih bolesnika i 50 zdravih ispitanika u razdoblju od siječnja 2016. do srpnja 2017. godine u Klinici za dermatovenerologiju KBC Zagreb. Za određivanje mutacije filagrinskog gena korišten je bukalni bris. Uzorkovanjem SC-a uzet je materijal za određivanje NMF-a, citokina i lipida. Funkcija barijere je mjerena određivanjem NMF-a, TEWL-a, pH i SA. Nađena je niska učestalost filagrinskih mutacija (4%) i normalne razine filagrinskih degradacijskih produkata čime zaključujemo da nedostatak filagrina ne igra značajnu ulogu u etiologiji AD-a u hrvatskoj populaciji. Lipidi pokazuju značajne razlike između bolesnika i zdravih ispitanika i povezanost s težinom bolesti i funkcijom kožne barijere. Promjene su izraženije u lezionalnoj koži. Određivanje imunoloških markera potvrdilo je prisutnost velikog broja citokina prirođene i stečene imunosti. Najznačajnija razlika između zdrave i kože bolesnika nađena je za citokine prirođene imunosti IL-18 i CXCL8, ali značajne razlike nađene su i za Th1, Th2, Th17 i Th22 citokine. Razina citokina povezana je s težinom bolesti i funkcijom kožne barijere, a za razliku od lipida povezanost je izraženija u nelezionalnoj koži. Provedena studija pokazuje pogodnost SC u određivanje širokog spektra biomarkera koji mogu doprinijeti razvoju ciljane terapije prilagođene bolesniku.
Abstract (english)
We studied genotype and phenotype biomarkers of epidermal barrier in atopic dermatitis (AD) patients. Study population included 150 individuals, 100 patients and 50 healthy controls from 2016 to 2017 at University Department of Dermatovenereology Clinical Hospital Centre Zagreb. Buccal swab was used for genotypization and the other biomarkers were determinated in stratum corneum (SC). We found low prevalence of filaggrin mutations (4%) and normal levels of filaggrin degradation products suggesting that filaggrin does not play major role in pathogenesis of AD in our cohort. Lipid levels are significantly changed when compared AD skin and healthy skin, and significant correlation with disease severity and barrier function was found. More prominent changes were found in lesional skin suggesting that majority of observed changes are secondary due to inflammation. We determinated great number of cytokines of innate and adaptive immune response. The most significant difference between healthy and AD skin was found for innate imunity cytokines IL-18 and CXCL8, but significant response was found for also for Th1, Th2, Th17 and Th22. Cytokine levels were associated with disease severity and barrier function, and opposite to lipids, correlation was more prominent in non-lesional skin. This study confirmed that SC is suitable for determination of wide range of biomarkers which can contribute for development of precise, patient-based therapy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:875771
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Catalog URLhttps://katalog.nsk.hr/F/19IT7Y755V1NXQJPHCLYSQGPF6BLAPPF179HQ7FAXXMYBYGMV5-09268?func=find-e&request=Odre%C4%91ivanje+genotipskih+i+fenotipskih+biomarkera+ko%C5%BEne+barijere+u+bolesnika+s+atopijskim+dermatitisom&find_scan_code=FIND_NAS&adjacent=N&local_base=MF_WEB&x=46&y=7&filter_code_1=WLN&filter_request_1=&filter_code_2=WYR&filter_request_2=&filter_code_3=WYR&filter_request_3=&filter_code_4=WFM&filter_request_4=
Type of resourceText
Extent95 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-03-23 09:16:07