prikaz prve stranice dokumenta Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis
  Download
PDF 2.07 MB
Scientific paper - Original scientific paper
Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis
Clinical and experimental rheumatology, 38 (2020), 5; 903-916. urn:nbn:hr:105:029043
Šućur, Alan; Jajić, Zrinka; Ikić Matijašević, Marina; Stipić Marković, Asja; Flegar, Darja; Lukač, Nina; Kelava, Tomislav; Kovačić, Nataša; Grčević, Danka

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Šućur, A., Jajić, Z., Ikić Matijašević, M., Stipić Marković, A., Flegar, D., Lukač, N. ... Grčević, D. (2020). Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis. Clinical and experimental rheumatology, 38. (5), 903-916. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:029043

Šućur, Alan, et al. "Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis." Clinical and experimental rheumatology, vol. 38, no. 5, 2020, pp. 903-916. https://urn.nsk.hr/urn:nbn:hr:105:029043

Šućur, Alan, Zrinka Jajić, Marina Ikić Matijašević, Asja Stipić Marković, Darja Flegar, Nina Lukač, Tomislav Kelava, Nataša Kovačić and Danka Grčević. "Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis." Clinical and experimental rheumatology 38, no. 5 (2020): 903-916. https://urn.nsk.hr/urn:nbn:hr:105:029043

Šućur, A., et al. (2020) 'Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis', Clinical and experimental rheumatology, 38(5), pp. 903-916. Available at: https://urn.nsk.hr/urn:nbn:hr:105:029043 (Accessed 29 November 2024)

Šućur A, Jajić Z, Ikić Matijašević M, Stipić Marković A, Flegar D, Lukač N, and sur.. Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis. Clinical and experimental rheumatology [Internet]. 2020 October [cited 2024 November 29];38(5):903-916. Available at: https://urn.nsk.hr/urn:nbn:hr:105:029043

A. Šućur, et al., "Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis", Clinical and experimental rheumatology, vol. 38, no. 5, pp. 903-916, October 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:029043. [Accessed: 29 November 2024]

Metadata
Title (english)Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis
AuthorAlan Šućur
AuthorZrinka Jajić
AuthorMarina Ikić Matijašević
AuthorAsja Stipić Marković
AuthorDarja Flegar
AuthorNina Lukač
AuthorTomislav Kelava
AuthorNataša Kovačić
AuthorDanka Grčević
Author's institutionUniversity of Zagreb
School of Medicine
(Croatian Institiute for Brain Research)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Anatomy and Clinical Anatomy)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Physical Medicine and General Rehabilitation)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Physiology and Immunology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Anatomy
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Physical Medicine and Rehabilitation
Abstract (english)
Objectives: Rheumatoid arthritis (RA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA) are associated with abnormal immune cell functions. We combined manual and automated profiling in subpopulations of T-cells, B-cells and monocytes, in parallel to functional testing and clinical correlation. ----- Methods: Using flow cytometry, we analysed the expression of CCR4, CCR6 and CXCR5 on helper and cyotoxic T-cells, CD32B and CD86 on naïve and memory B-cells, and CCR1, CCR2, CCR4 and CXCR4 on monocytes in chronic high-disease activity patients to identify peripheral blood subpopulations. Cell activation, proliferative capability and osteoclastogenic effects were tested in vitro. Comparison with synovial compartment, clinical data and anti-TNF treatment were added to peripheral blood analysis. ----- Results: PsA had lower double-negative T-cell frequency, while RA had lower double-positive T-cell frequency and expanded Th1-like and cytotoxic T-cell subsets. CD32B expression was increased on naïve and memory B-cells in AS and associated with disease activity. CCR6+ and CXCR5+ cytotoxic T-cells and CD32B+ naïve and memory B-cells were highly enriched within the synovial compartment. T-cells and B-cells from AS exhibited enhanced activation and proliferation in vitro, whereas T-cell conditioned medium from RA produced an increased osteoclastogenic effect. CCR1 and CXCR4 were upregulated on osteoclastogenic monocyte subsets of RA, AS and PsA patients. Bioinformatic Citrus analysis identified additional T-cell, B-cell and monocyte clusters specifically associated with each disease. ----- Conclusions: By combining manual and automated data analysis, our study revealed several disease-specific immune cell subpopulations, particularly cytotoxic T-cell subsets in RA and memory B-cell subsets in AS, which may serve as an indicator of active disease or possible therapeutic target.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleClinical and experimental rheumatology
Numberingvol. 38, no. 5, pp. 903-916
p-ISSN0392-856X
e-ISSN1593-098X
URN:NBNurn:nbn:hr:105:029043
Publication2020-10
Document URLhttps://www.clinexprheumatol.org/abstract.asp?a=14516
Type of resourceText
Access conditionsOpen access
Embargo expiration date: 2021-11-01
Terms of useLicence In copyright icon
Created on2021-05-24 07:57:22