prikaz prve stranice dokumenta Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice
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doctoral thesis
Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice
Zagreb: University of Zagreb, School of Medicine, 2021. urn:nbn:hr:105:524607
Smilović, Dinko
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Smilović, D. (2021). Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:524607

Smilović, Dinko. "Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice." Doctoral thesis, University of Zagreb, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:105:524607

Smilović, Dinko. "Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice." Doctoral thesis, University of Zagreb, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:105:524607

Smilović, D. (2021). 'Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice', Doctoral thesis, University of Zagreb, School of Medicine, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:524607

Smilović D. Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2021 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:105:524607

D. Smilović, "Morphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:105:524607

Metadata
TitleMorphological analysis of the dendritic tree and the expression and localization of actin-modulating protein synaptopodin in hippocampal granule cells of TNF-α KO mice
Title (croatian)Morfološka analiza dendritičkog stabla te izražaj i lokalizacija aktin-modulirajućeg proteina synaptopodina u hipokampalnim zrnatim stanicama TNF-α-KO miševa.
AuthorDinko Smilović
MentorMario Vukšić (mentor)
Committee memberZdravko Petanjek (predsjednik povjerenstva)
Committee memberGoran Šimić (član povjerenstva)
Committee memberIvica Kostović (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2021-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Changes in the morphology of dendrites and dendritic spines are tightly correlated to the functional properties of neurons. The underlying mechanisms are under strict control by multiple effectors. One of such proteins, the tumor necrosis factor α (TNF-α) has been shown to influence synaptic transmission and plasticity and is tightly connected to the actin-modulating protein synaptopodin (SP). We performed a detailed analysis of dendritic arbors and dendritic spines of dentate granule cells in mice mutants lacking TNF-α or its receptors. Fixed hippocampal sections were double-stained for SP, a molecular marker for strong and stable spines. We have shown that granule cells of TNF-deficient mice have fewer deficits in dendritic tree arborization after denervation. Dendritic spine density was reduced by 20% in these mice, and a compensatory increase in the size of large, SP+ spines was seen. A strikingly similar pattern of changes was seen in mice lacking TNF-R1, but not in mice lacking TNF-R2, showcasing that TNF-R1 is the dominant effector for this mechanism. Genetic removal of both receptors caused a large, 28% reduction in average spine sizes, and the compensatory, homeostatic increase of large spine sizes and SP content was absent.
Abstract (croatian)
Promjene u morfologiji dendrita i dendritičkih trnova usko su povezane s funkcionalnim svojstvima neurona. Osnovni mehanizmi tih promjena su pod strogom kontrolom mnogih molekula. Pokazalo se da jedan od takvih proteina, faktor tumorske nekroze α (TNF-α) utječe na sinaptički prijenos i plastičnost te je usko povezan s aktin-modulirajućim proteinom synaptopodinom (SP). U ovom radu smo detaljno analizirali dendritičko stablo i dendritičke trnove zrnatih stanica girusa dentatusa u miševa mutanata kojima nedostaje gen za TNF-α ili njegovi receptori. Fiksirane hipokampalne kriške su bile imunohistokemijski obojene za SP, molekularni marker stabilnih i snažnih dendritičkih trnova. Pokazali smo da zrnate stanice miševa kojima nedostaje gen za TNF-α imaju manje promjena u grananju dendritičkog stabla nakon denervacije. Gustoća dendritičkih trnova je smanjena za 20% kod tih miševa, a uočen je nadomjesni porast veličine velikih SP+ dendritičkih trnova. Zapanjujuće, sličan obrazac promjena viđen je kod miševa kojima nedostaje gen za TNF-R1, ali ne i kod miševa kojima nedostaje gen za TNF-R2, ukazujući na to da je TNF-R1 odlučujući čimbenik u nastanku ovih promjena. Genetsko uklanjanje oba receptora prouzročilo je veliko, 28% smanjenje prosječnih veličina dendritičkih trnova, te je u tim miševima izostao kompenzacijski, homeostatski porast velikih dendritičkih trnova, kao i sadržaja SP.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:105:524607
Promotion2021
ProjectNumber: KK.01.1.1.01.0007
Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Leader: Ivica Kostović
Jurisdiction: Croatia
Funder: EK
Study programmeTitle: Neuroscience
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent115 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteSumentor: Thomas Deller
Created on2021-08-03 07:19:13