Secreted frizzled-related protein (SFRP) family is the largest family of Wnt signaling pathway. Current research efforts clearly outline the relationship between the decreased SFRP protein expression and develepement of colorectal and urothelial carcinomas. The highest percentages of testicular tumors are of those germline origin, among which seminomas represent the most common histotype. Taking into account that SFRP proteins act as negative regulators of Wnt signaling pathway, the purpose of this work was to determine the SFRP1 and SFRP3 protein expression in a defined cohort of primary testicular tumors (including both seminomas and non-seminomas/mixed germ cell), using immunohistochemistry. Tumor samples were collected along with the adjacent normal/benign tissue that was used as a control sample. Protein expressions were measured and the results were semiquantitively analyzed and statistically processed. Obtain results showed statistically significant difference in expression of SFRP1 (seminomas=0.1 (0.7), non-seminomas=0.1 (0.6), controls=1 (0), p<0.001) and SFRP3 proteins (seminomas=0.003 (0.3), non-seminomas=0.05 (0.7), controls=1 (0) p<0.001) between tumorous and healthy (control) tissue. The difference in expression between particular histological subtypes of tumors has not being found. Moreover, there was no significant difference between the expression of SFRP1 and SFRP3 protein in respect to patients age. We conclude that decreased expression of Wnt signaling regulators SFRP1 and SFRP3 can be observed in testicular germ cell tumors compared with normal tissue. These findings suggest their possible role in testicular germline tumorigenesis.