Infertility in couples is common and has become a global burden, remaining high over the years. The National Institute for Health and Care Excellence (NICE) recommends that prolonged or unresolved infertility should be treated using assisted reproduction technology. The purpose of this study was to evaluate the impact of elevated day-2 serum progesterone levels on IVF and ICSI outcome regarding pregnancy. A one-center cohort retrospective study was conducted during the period of February 2020 to May 2020. The study includes data from 136 patients who underwent a total of 189 fresh embryo transfers in IVF/ICSI antagonist cycles from January 2014 to January 2020. Based on the levels of day-2 serum progesterone the total number of cases (n=189) were divided into two groups; group 1 (n=134) with serum progesterone level of ≤1.5 ng/mL (normal) and group 2 (n=55) with serum progesterone level of >1.5 ng/mL (high). The total dose of gonadotropins, age, BMI, AMH, FSH, LH, PRL, the number of oocytes retrieved, the number of embryos transferred and frozen, and positive pregnancy tests were compared between the two groups. The results showed that there was no statistically significant difference between age, BMI, AMH, FSH, LH, PRL, total dose of gonadotropins, the number of oocytes retrieved, and the technique used (IVF or ICSI) between the two groups. As well there was no statistically significant difference between the two groups in regards to the number of embryos transferred and the number of frozen embryos. Although a higher percentage of pregnancies based on positive hCG was found in the normal progesterone level group (33.6%) than in the high progesterone group (23.8%), there was no statistically significant difference between the two groups (p-value 0.178). In conclusion, this study found that an elevated day-2 serum progesterone level does not have a significant impact on the clinical outcome of IVF/ICSI antagonist cycles with fresh embryo transfers.