prikaz prve stranice dokumenta Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
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Scientific paper - Original scientific paper
Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
International Journal of Molecular Sciences, 22 (2021), 24; 13590. https://doi.org/10.3390/ijms222413590
Ilić, Katarina; Lin, Xiao; Malci, Ayse; Stojanović, Mario; Puljko, Borna; Rožman, Marko; Vukelić, Željka; Heffer, Marija; Montag, Dirk; Schnaar, Ronald L.; Kalanj-Bognar, Svjetlana; Herrera-Molina, Rodrigo; Mlinac-Jerković, Kristina

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Ilić, K., Lin, X., Malci, A., Stojanović, M., Puljko, B., Rožman, M. ... Mlinac Jerković, K. (2021). Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. International Journal of Molecular Sciences, 22. (24). doi: 10.3390/ijms222413590

Ilić, Katarina, et al. "Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts." International Journal of Molecular Sciences, vol. 22, no. 24, 2021. https://doi.org/10.3390/ijms222413590

Ilić, Katarina, Xiao Lin, Ayse Malci, Mario Stojanović, Borna Puljko, Marko Rožman, Željka Vukelić, et al. "Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts." International Journal of Molecular Sciences 22, no. 24 (2021). https://doi.org/10.3390/ijms222413590

Ilić, K., et al. (2021) 'Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts', International Journal of Molecular Sciences, 22(24). doi: 10.3390/ijms222413590

Ilić K, Lin X, Malci A, Stojanović M, Puljko B, Rožman M, and sur.. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. International Journal of Molecular Sciences [Internet]. 2021 December 18 [cited 2024 December 27];22(24). doi: 10.3390/ijms222413590

K. Ilić, et al., "Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts", International Journal of Molecular Sciences, vol. 22, no. 24, December 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:896617. [Accessed: 27 December 2024]

Metadata
Title (english)Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
AuthorKatarina Ilić
AuthorXiao Lin
AuthorAyse Malci
AuthorMario Stojanović
AuthorBorna Puljko
AuthorMarko Rožman
AuthorŽeljka Vukelić
AuthorMarija Heffer
AuthorDirk Montag
AuthorRonald L. Schnaar
AuthorSvjetlana Kalanj-Bognar
AuthorRodrigo Herrera-Molina
AuthorKristina Mlinac-Jerković
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Chemistry, Biochemistry and Clinical Chemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The recent identification of plasma membrane (Ca2+)-ATPase (PMCA)-Neuroplastin (Np) complexes has renewed attention on cell regulation of cytosolic calcium extrusion, which is of particular relevance in neurons. Here, we tested the hypothesis that PMCA-Neuroplastin complexes exist in specific ganglioside-containing rafts, which could affect calcium homeostasis. We analyzed the abundance of all four PMCA paralogs (PMCA1-4) and Neuroplastin isoforms (Np65 and Np55) in lipid rafts and bulk membrane fractions from GM2/GD2 synthase-deficient mouse brains. In these fractions, we found altered distribution of Np65/Np55 and selected PMCA isoforms, namely PMCA1 and 2. Cell surface staining and confocal microscopy identified GM1 as the main complex ganglioside co-localizing with Neuroplastin in cultured hippocampal neurons. Furthermore, blocking GM1 with a specific antibody resulted in delayed calcium restoration of electrically evoked calcium transients in the soma of hippocampal neurons. The content and composition of all ganglioside species were unchanged in Neuroplastin-deficient mouse brains. Therefore, we conclude that altered composition or disorganization of ganglioside-containing rafts results in changed regulation of calcium signals in neurons. We propose that GM1 could be a key sphingolipid for ensuring proper location of the PMCA-Neuroplastin complexes into rafts in order to participate in the regulation of neuronal calcium homeostasis.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 22, no. 24, 13590
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms222413590
URN:NBNurn:nbn:hr:105:896617
Publication2021-12-18
ProjectNumber: IP-2016-06-8636
Title (croatian): Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga
Acronym: NeuroReact
Leader: Svjetlana Kalanj-Bognar
Jurisdiction: Croatia
Funding stream: IP
ProjectNumber: IP-2014-09-2324
Title (croatian): Patofiziološke posljedice promjena sastava lipidnih splavi
Title (english): Pathophysiological consequences of lipid raft changes
Acronym: RafTuning
Jurisdiction: Croatia
Funding stream: IP
Document URLhttps://www.mdpi.com/1422-0067/22/24/13590
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-02-01 09:24:09