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Scientific paper - Original scientific paper
Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
World Journal of Methodology, 11 (2021), 6; 294-301. https://doi.org/10.5662/wjm.v11.i6.294
Vilibić-Čavlek, Tatjana; Barbić, Ljubo; Stevanović, Vladimir; Savić, Vladimir; Mrzljak, Anna; Bogdanić, Maja; Tabain, Irena

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Vilibić Čavlek, T., Barbić, Lj., Stevanović, V., Savić, V., Mrzljak, A., Bogdanić, M. & Tabain, I. (2021). Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections. World Journal of Methodology, 11. (6), 294-301. doi: 10.5662/wjm.v11.i6.294

Vilibić Čavlek, Tatjana, et al. "Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections." World Journal of Methodology, vol. 11, no. 6, 2021, pp. 294-301. https://doi.org/10.5662/wjm.v11.i6.294

Vilibić Čavlek, Tatjana, Ljubo Barbić, Vladimir Stevanović, Vladimir Savić, Anna Mrzljak, Maja Bogdanić and Irena Tabain. "Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections." World Journal of Methodology 11, no. 6 (2021): 294-301. https://doi.org/10.5662/wjm.v11.i6.294

Vilibić Čavlek, T., et al. (2021) 'Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections', World Journal of Methodology, 11(6), pp. 294-301. doi: 10.5662/wjm.v11.i6.294

Vilibić Čavlek T, Barbić Lj, Stevanović V, Savić V, Mrzljak A, Bogdanić M, and sur.. Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections. World Journal of Methodology [Internet]. 2021 November 20 [cited 2024 November 23];11(6):294-301. doi: 10.5662/wjm.v11.i6.294

T. Vilibić Čavlek, et al., "Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections", World Journal of Methodology, vol. 11, no. 6, pp. 294-301, November 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:391304. [Accessed: 23 November 2024]

Metadata
Title (english)Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
AuthorTatjana Vilibić-Čavlek
AuthorLjubo Barbić
AuthorVladimir Stevanović
AuthorVladimir Savić
AuthorAnna Mrzljak
AuthorMaja Bogdanić
AuthorIrena Tabain
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Internal Medicine)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract (english)
Background: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. ----- Aim: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. ----- Methods: One hundred eighty-eight serum samples from Puumala (PUUV) and Dobrava (DOBV) orthohantavirus infected patients were analyzed. Serology was performed using commercial tests (Euroimmun, Lübeck, Germany). ----- Results: Using IFA, 49.5% of acute-phase samples showed a monotypic response to PUUV, while 50.5% cross-reacted with other hantaviruses. The overall cross-reactivity was higher for immunoglobulin G (IgG) (50.0%) than for immunoglobulin M (IgM) (25.5%). PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan (12.3%/31.5%), Seoul (7.5%/17.8%), DOBV (5.4%/ 28.1%), and Saaremaa (4.8%/15.7%). Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan (76.2%/95.2%), Saaremaa (80.9%/83.3%), and Seoul (78.6%/85.7%) and moderate with PUUV (28.5%/38.1%). Using a WB, serotyping was successful in most cross-reactive samples (89.5%). ----- Conclusion: The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections, confirming serotype in most IFA cross-reactive samples.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleWorld Journal of Methodology
Numberingvol. 11, no. 6, pp. 294-301
p-ISSN2222-0682
DOIhttps://doi.org/10.5662/wjm.v11.i6.294
URN:NBNurn:nbn:hr:105:391304
Publication2021-11-20
Document URLhttps://www.wjgnet.com/2222-0682/full/v11/i6/294.htm
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) icon
Created on2022-02-03 09:17:54