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master's thesis
Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka
Zagreb: University of Zagreb, School of Medicine, 2014. urn:nbn:hr:105:715092
Marčinković, Nedo
University of Zagreb
School of Medicine
Department of Forensic Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Marčinković, N. (2014). Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:715092

Marčinković, Nedo. "Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka." Master's thesis, University of Zagreb, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:105:715092

Marčinković, Nedo. "Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka." Master's thesis, University of Zagreb, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:105:715092

Marčinković, N. (2014). 'Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka', Master's thesis, University of Zagreb, School of Medicine, accessed 20 May 2024, https://urn.nsk.hr/urn:nbn:hr:105:715092

Marčinković N. Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2014 [cited 2024 May 20] Available at: https://urn.nsk.hr/urn:nbn:hr:105:715092

N. Marčinković, "Utvrđivanje očinstva analizom DNA iz posmrtnih ostataka", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:105:715092

Metadata
TitleUtvrđivanje očinstva analizom DNA iz posmrtnih ostataka
Title (english)Paternity determination with DNA analysis of posthumous remains
AuthorNedo Marčinković
MentorMilovan Kubat (mentor)
Committee memberDavor Strinović (predsjednik povjerenstva)
Committee memberDavor Mayer (član povjerenstva)
Committee memberMilovan Kubat (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Forensic Medicine)
Zagreb
Defense date and country2014-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Forensic Medicine
Abstract
Metode utvrđivanja očinstva,a posebice onih iz posmrtnih ostataka izuzetno su napredovale posljednjih desetljeća. Sve je krenulo još sredinom 80-ih godina izolacijom molekule DNA, nastavilo se mapiranjem humanog genoma, a rezultiralo da danas sa 99,9999 %-tnom vjerojatnošću možemo utvrditi da li je pokojni, otac djeteta. Prvi korak u utvrđivanju očinstva je prikupljanje odgovarajućeg uzorka. U funkciji uzorka su najčešće krv, tkivo i kost pokojnog. Iz navedenih uzoraka potrebno je izolirati molekulu DNA koja će nam poslužiti za usporedbu s DNA potencijalnog djeteta i njegove majke. Nakon obrade materijala i dobivanja uzorka DNA uzima se mali segment tj. STR koji se amplificira uz pomoć PCR metode. Na taj način i iz iznimno male količine genetskog materijala možemo dobiti enormne količine uzorka za usporedbu. Usporedbom STR segmenata posmrtnih ostataka potencijalnog oca sa onima djeteta moguće je sa 99,9999%-tnom vjerojatnošću utvrditi očinstvo ili isto isključiti ako postoji više od pet STR nepodudarnosti.
Abstract (english)
Methods of paternal identification especially ones performed from posthumous remains improved rapidly in last few decades. Everything begun in 80's with isolation of DNA molecule, continued with human genome mapping, and resulted that nowdays we can determine whether deceased is father of a child with 99,9999% accuracy. First step in paternal identification is collecting adequate samples.Sample is usually blood, tissue and bone of deceased. We use samples to isolate DNA molecule which will be used to compare with DNA of potential child and it's mother. After thorrow processing the samples and isolating DNA we need a samll fragment called STR which is multiplied by PCR method. On that way even with small amounts of genetic material we can get enormous amounts of sample for comparison. Comparing STR fragments of the deceased with ones from the child and it's mother we can make paternal identification within 99,9999% accuracy or exclude one if there is more than five STR expulsion.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:715092
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2015-09-25 12:32:30