Healthcare-associated infections (HAI) are a problem for many patients, and especially for newborns admitted to intensive care units. Types of HAI include those associated with the use of intravascular catheters. Risk factors for these infections include an immature immune and gastrointestinal system, diagnostic and therapeutic procedures, and impaired skin barrier function. CR-BSI (catheter-related bloodstream infection) means catheter-related infection that is proven by laboratory testing. Prevention of infections of this type is based on understanding the mechanisms of infection, the development of bacterial biofilm, the possibility of spreading microorganisms using a central venous catheter and prevention methods. The most important factor in preventing the transmission of microorganisms is hand hygiene and the application of aseptic techniques during the insertion of catheters and during their manipulation in order to reduce the possibility of transmission of microorganisms. "Five moments for hand hygiene" means key moments in implementation of hand disinfection methods. The use of skin antiseptics is one of the most important step in preventing the occurrence of infection, however, data on the safety of antiseptics on the skin of the newborn are scarce so it is necessary to use them with caution. In order to prevent infections caused by central catheters, guidelines have been developed in the form of care bundles, which, through systematic application, contribute to reducing the occurrence of infections.